Engineering a nanopore with co-chaperonin function.
Authors
Ho, Ching-Wen [3]; Van Meervelt, Veerle [4]; Tsai, Keng-Chang [5]; Pieter-Jan De Temmerman [6]; Jan Mast [7]; Maglia, Giovanni [8]Keywords
Abstract:
The emergence of an enzymatic function can reveal functional insights and allows the engineering of biological systems with enhanced properties. We engineered an alpha hemolysin nanopore to function as GroES, a protein that, in complex with GroEL, forms a two-stroke protein-folding nanomachine. The transmembrane co-chaperonin was prepared by recombination of GroES functional elements with the nanopore, suggesting that emergent functions in molecular machines can be added bottom-up by incorporating modular elements into preexisting protein scaffolds. The binding of a single-ring version of G…