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Home > Biblio > Fast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization-time of flight mass spectrometry: validation in the clinical laboratory.

Fast and accurate identification of dermatophytes by matrix-assisted laser desorption ionization-time of flight mass spectrometry: validation in the clinical laboratory. [1]

Health and environment   [2]

Peer reviewed scientific article

SCIENSANO

Authors

Ann Packeu [3]; De Bel, A [4]; l'Ollivier, C [5]; Ranque, S [6]; Detandt, M [7]; Marijke Hendrickx [8]

Keywords

  1. Arthrodermataceae [9]
  2. Clinical Laboratory Techniques [10]
  3. Dermatomycoses [11]
  4. Humans [12]
  5. specimen handling [13]
  6. Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization [14]
  7. Time Factors [15]

Abstract:

The performance of a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) workflow using an extensive reference database for dermatophyte identification was evaluated on 176 clinical strains. Using a direct-deposit procedure after 3 incubation days yielded 40% correct identification. Both increasing incubation time and using an extraction procedure resulted in 100% correct identification.
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Associated health topics:

Health and environment [2]
Source URL: https://www.sciensano.be/en/biblio/fast-and-accurate-identification-dermatophytes-matrix-assisted-laser-desorption-ionization-time

Links
[1] https://www.sciensano.be/en/biblio/fast-and-accurate-identification-dermatophytes-matrix-assisted-laser-desorption-ionization-time
[2] https://www.sciensano.be/en/health-topics/health-and-environment
[3] https://www.sciensano.be/en/people/ann-packeu/biblio
[4] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=49056&f%5Bsearch%5D=De%20Bel%2C%20A
[5] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=49059&f%5Bsearch%5D=l%27Ollivier%2C%20C
[6] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=36690&f%5Bsearch%5D=Ranque%2C%20S
[7] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=48966&f%5Bsearch%5D=Detandt%2C%20M
[8] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=36810&f%5Bsearch%5D=Marijke%20Hendrickx
[9] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=19284&f%5Bsearch%5D=Arthrodermataceae
[10] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=4080&f%5Bsearch%5D=Clinical%20Laboratory%20Techniques
[11] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=20094&f%5Bsearch%5D=Dermatomycoses
[12] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=648&f%5Bsearch%5D=Humans
[13] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=5463&f%5Bsearch%5D=specimen%20handling
[14] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=32364&f%5Bsearch%5D=Spectrometry%2C%20Mass%2C%20Matrix-Assisted%20Laser%20Desorption-Ionization
[15] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=3618&f%5Bsearch%5D=Time%20Factors