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Home > Biblio > Method for fluorescent marker swapping and its application in Steinernema nematode colonization studies.

Method for fluorescent marker swapping and its application in Steinernema nematode colonization studies. [1]

Animal health   [2]

Peer reviewed scientific article

SCIENSANO

Authors

Gengler, Samuel [3]; Batoko, Henri [4]; P Wattiau [5]

Keywords

  1. alleles [6]
  2. Animals [7]
  3. Genetic Vectors [8]
  4. Green Fluorescent Proteins [9]
  5. Luminescent Proteins [10]
  6. Microscopy, Confocal [11]
  7. Rhabditida [12]
  8. symbiosis [13]
  9. Xenorhabdus [14]
  10. Yersinia pseudotuberculosis [15]

Abstract:

An allelic exchange vector was constructed to replace gfp by mCherry in bacteria previously tagged with mini-Tn5 derivatives. The method was successfully applied to a gfp-labeled Yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization of Steinernema nematodes hosting their Xenorhabdus symbiont using dual-color confocal microscopy.
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Associated health topics:

Animal health [2]
Source URL:https://www.sciensano.be/en/biblio/method-fluorescent-marker-swapping-and-its-application-steinernema-nematode-colonization-studies

Links
[1] https://www.sciensano.be/en/biblio/method-fluorescent-marker-swapping-and-its-application-steinernema-nematode-colonization-studies [2] https://www.sciensano.be/en/health-topics/animal-health [3] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=39780&f%5Bsearch%5D=Gengler%2C%20Samuel [4] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=39786&f%5Bsearch%5D=Batoko%2C%20Henri [5] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=39180&f%5Bsearch%5D=P%20Wattiau [6] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2904&f%5Bsearch%5D=alleles [7] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=423&f%5Bsearch%5D=Animals [8] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=21171&f%5Bsearch%5D=Genetic%20Vectors [9] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=29514&f%5Bsearch%5D=Green%20Fluorescent%20Proteins [10] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=23514&f%5Bsearch%5D=Luminescent%20Proteins [11] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=29517&f%5Bsearch%5D=Microscopy%2C%20Confocal [12] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=29709&f%5Bsearch%5D=Rhabditida [13] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=4188&f%5Bsearch%5D=symbiosis [14] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=29712&f%5Bsearch%5D=Xenorhabdus [15] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=29706&f%5Bsearch%5D=Yersinia%20pseudotuberculosis