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Home > Biblio > Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.

Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies. [1]

Animal health   [2]

Peer reviewed scientific article

SCIENSANO

Authors

Bréard, Emmanuel [3]; Lara, Estelle [4]; Comtet, Loïc [5]; Viarouge, Cyril [6]; Doceul, Virginie [7]; Desprat, Alexandra [8]; Vitour, Damien [9]; Pozzi, Nathalie [10]; Ann Brigitte Cay [11]; Nick De Regge [12]; Pourquier, Philippe [13]; Schirrmeier, Horst [14]; Hoffmann, Bernd [15]; Beer, Martin [16]; Sailleau, Corinne [17]; Zientara, Stéphan [18]

Keywords

  1. Animals [19]
  2. Antibodies, Neutralizing [20]
  3. Antibodies, Viral [21]
  4. Bunyaviridae Infections [22]
  5. Cattle [23]
  6. Enzyme-Linked Immunosorbent Assay [24]
  7. Europe [25]
  8. Fluorescent Antibody Technique, Indirect [26]
  9. Gene Expression [27]
  10. Neutralization Tests [28]
  11. Nucleocapsid Proteins [29]
  12. Orthobunyavirus [30]
  13. Reagent Kits, Diagnostic [31]
  14. Recombinant Proteins [32]
  15. Reproducibility of Results [33]
  16. Roc Curve [34]
  17. Sheep [35]

Abstract:

A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine co…
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Associated health topics:

Animal health [2]
Source URL:https://www.sciensano.be/en/biblio/validation-a-commercially-available-indirect-elisa-using-a-nucleocapside-recombinant-protein

Links
[1] https://www.sciensano.be/en/biblio/validation-a-commercially-available-indirect-elisa-using-a-nucleocapside-recombinant-protein [2] https://www.sciensano.be/en/health-topics/animal-health [3] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=510&f%5Bsearch%5D=Br%C3%A9ard%2C%20Emmanuel [4] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=40332&f%5Bsearch%5D=Lara%2C%20Estelle [5] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=40335&f%5Bsearch%5D=Comtet%2C%20Lo%C3%AFc [6] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=38082&f%5Bsearch%5D=Viarouge%2C%20Cyril [7] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=40338&f%5Bsearch%5D=Doceul%2C%20Virginie [8] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=40341&f%5Bsearch%5D=Desprat%2C%20Alexandra [9] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=38106&f%5Bsearch%5D=Vitour%2C%20Damien [10] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=40344&f%5Bsearch%5D=Pozzi%2C%20Nathalie [11] https://www.sciensano.be/en/people/brigitte-cay/biblio [12] https://www.sciensano.be/en/people/nick-de-regge/biblio [13] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=38442&f%5Bsearch%5D=Pourquier%2C%20Philippe [14] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=40347&f%5Bsearch%5D=Schirrmeier%2C%20Horst [15] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=37752&f%5Bsearch%5D=Hoffmann%2C%20Bernd [16] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=37755&f%5Bsearch%5D=Beer%2C%20Martin [17] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=38109&f%5Bsearch%5D=Sailleau%2C%20Corinne [18] https://www.sciensano.be/en/biblio?f%5Bauthor%5D=37863&f%5Bsearch%5D=Zientara%2C%20St%C3%A9phan [19] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=423&f%5Bsearch%5D=Animals [20] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=27885&f%5Bsearch%5D=Antibodies%2C%20Neutralizing [21] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=441&f%5Bsearch%5D=Antibodies%2C%20Viral [22] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=27738&f%5Bsearch%5D=Bunyaviridae%20Infections [23] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=426&f%5Bsearch%5D=Cattle [24] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=432&f%5Bsearch%5D=Enzyme-Linked%20Immunosorbent%20Assay [25] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=42&f%5Bsearch%5D=Europe [26] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=29193&f%5Bsearch%5D=Fluorescent%20Antibody%20Technique%2C%20Indirect [27] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=5475&f%5Bsearch%5D=Gene%20Expression [28] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=27888&f%5Bsearch%5D=Neutralization%20Tests [29] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=30255&f%5Bsearch%5D=Nucleocapsid%20Proteins [30] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=27744&f%5Bsearch%5D=Orthobunyavirus [31] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=29724&f%5Bsearch%5D=Reagent%20Kits%2C%20Diagnostic [32] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=3657&f%5Bsearch%5D=Recombinant%20Proteins [33] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=2397&f%5Bsearch%5D=Reproducibility%20of%20Results [34] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=19056&f%5Bsearch%5D=Roc%20Curve [35] https://www.sciensano.be/en/biblio?f%5Bkeyword%5D=459&f%5Bsearch%5D=Sheep