Search results - 25 results
Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains.
difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed and validated a new duplex real-time PCR capable of ...
Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease.
that can be used during assay development and in validation exercises to compare the performance of these varied (and sometimes competing) diagnostic technologies. World Organisation for Animal Health ...
Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease
Revue Scientifique et Technique de l'OIE, Volume 40, Issue 1 (2021) Keywords: Assay validation Case study Diagnostic testing Foot and mouth disease Foot and mouth disease vaccine Gaps ...
The African swine fever virus isolate Belgium 2018/1 shows high virulence in European wild boar.
The study also provided a large set of well-characterized sample materials for test validation and assay harmonization. Health Topics: Animal health Santé animale Diergezondheid Manuscript versions: ...
Development and validation of a TaqMan probe-based real-time PCR method for the differentiation of wild type lumpy skin disease virus from vaccine virus strains.
targeting the GPCR gene was developed and validated, for the concurrent detection and differentiation of wild type and vaccine Lumpy skin disease virus (LSDV) strains. The method was evaluated in three ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
addition of 5’-tails to the FMDV-specific primers enhances the detection of FMDV in both the 3D and the 5UTR RT-qPCR assay. To validate the 3D and 5UTR RT-qPCR assays with 5’-tailed primers for diagnostic ...
Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
Animals Antibodies, Neutralizing Antibodies, Viral Bunyaviridae Infections Cattle Enzyme-Linked Immunosorbent Assay Europe Fluorescent Antibody Technique, Indirect Gene Expression Neutralization Tests ...
Bayesian estimation of true prevalence, sensitivity and specificity of indirect ELISA, Rose Bengal Test and Slow Agglutination Test for the diagnosis of brucellosis in sheep and goats in Bangladesh.
Enzyme-Linked Immunosorbent Assay Goat Diseases Goats Immunoglobulins prevalence Rose Bengal Sensitivity and Specificity Serologic Tests Sheep Sheep Diseases Abstract: The true prevalence of brucellosis and ...
Evaluation of four enzyme-linked immunosorbent assays for the serologic survey of avian influenza in wild bird species.
More and more commercial enzyme-linked immunosorbent assays (ELISA) are available for serologic surveillance, but these tests are often developed and validated for use in domestic poultry. However, for ...
Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.
PCR methods recommended by OIE. This improved and rigorously validated real-time PCR assay with internal control will provide a rapid, sensitive and reliable molecular tool for ASFV detection in pigs in ...