Search results - 101 results

Establishment of the Ph. Eur. BRP for varicella vaccine batch 1.

monograph for varicella vaccine (live) (0648) requires a vial of an appropriate reference material to be titred in triplicate to validate each assay and the virus concentration of the reference preparation is ...

Validation of a liquid chromatography-mass spectrometric assay for tacrolimus in peripheral blood mononuclear cells.

validated in the range of 0.01-5 ng/mL. Limit of detection and of quantification are 0.005 and 0.01 ng/mL, respectively. Intra-assay and interassay recoveries ranged from 89.2% to 114.3% and 85.3% to 103.9%, ...

Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.

Salmonella kit uses a multiplexed DNA typing test aimed at identifying common serovars of Salmonella enterica. It was used in assays over a 9-month period in the Belgian reference laboratory that performs the ...

Indirect foot-and-mouth disease vaccine potency testing based on a serological alternative.

Immunosorbent Assay Foot-and-Mouth Disease Logistic Models Neutralization Tests Predictive Value of Tests Reproducibility of Results Roc Curve Statistics as Topic Viral Vaccines Abstract: Foot-and-mouth disease ...

Evaluation of antibody-ELISA and real-time RT-PCR for the diagnosis and profiling of bluetongue virus serotype 8 during the epidemic in Belgium in 2006.

Cattle Disease Outbreaks Enzyme-Linked Immunosorbent Assay Reverse Transcriptase Polymerase Chain Reaction Sheep Abstract: In 2006 bluetongue (BT) emerged for the first time in North-Western Europe. ...

Use of continuous results to compare ELISAs for the detection of antibodies to non-structural proteins of foot-and-mouth disease virus.

international workshop in Brescia, Italy in 2004 on the basis of dichotomous test results. However, as results from all of these assays were also available on a continuous scale, validation was extended by ...

An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen.

uninfected seronegative bulls, negative semen spiked with virus, as well as serial dilutions of reference virus. The samples were tested using a previously validated real-time PCR assay for the detection of ...

Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen.

detection of the presence of bovine herpesvirus type 1 (BoHV-1) in extended bovine semen. The assay detects a region encoding a highly conserved glycoprotein B gene. The real-time PCR assay was validated for ...

Validation of a liquid chromatography-mass spectrometric assay for tacrolimus in liver biopsies after hepatic transplantation: correlation with histopathologic staging of rejection.

Immunosuppressive Agents Liver Liver Transplantation Male middle aged Spectrometry, Mass, Electrospray Ionization Tacrolimus Tissue Distribution Abstract: The aims of this work were both to validate a sensitive and ...

A universal avian endogenous real-time reverse transcriptase-polymerase chain reaction control and its application to avian influenza diagnosis and quantification.

reverse transcriptase-polymerase chain reaction (RRT- PCR) is becoming an established first-line diagnostic assay as well as a precise quantification tool for avian influenza virus detection. However, there ...

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