Search results - 9 results

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

PCR marker was demonstrated by assays performed by an external laboratory. Health Topics:  Food consumption and food safety Consommation et sécurité alimentaires Voedselconsumptie en –veiligheid ...

A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products

fermentation products on the market is prohibited by European regulations. GMM s are currently screened for through qPCR assays targeting AMR genes and vectors, and then confirmed by targeting known specific GM ...

A genoserotyping system for a fast and objective identification of Salmonella serotypes commonly isolated from poultry and pork food sectors in Belgium.

their variants, subjected to an official control in the Belgian food sector. In this study, 3 additional assays using the same technology were developed for the rapid and cost-effective detection of 13 ...

Development of a genoserotyping system for the identification of Salmonella serotypes

biochemical test. From this molecular markers’ selection, 4 MOL- PCR assays were developed, i.e. the molecular markers were recognized by probes through a ligation-amplification reaction (MOL- PCR), followed by ...

Screening of endocrine activity of compounds migrating from plastic baby bottles using a multi-receptor panel of in vitro bioassays.

Demaegdt; Mertens, Birgit; Karine Vandermeiren; Scippo, Marie-Louise; Elskens, Marc Source: Toxicol In Vitro, Volume 37, p.121-133 (2016) Keywords: Animals Biological Assay Bottle Feeding Cell Line Cell Line, ...

Generation and Characterization of Six Recombinant Botulinum Neurotoxins as Reference Material to Serve in an International Proficiency Test.

concentration, catalytic and biological activities. For BoNT/A₁, B₁ and E₁, serotypes pathogenic to humans, the catalytic activity and the precise protein concentration were determined by Endopep-mass spectrometry ...

2,4-Dialkyl-8,9,10,11-tetrahydrobenzo[g]pyrimido[4,5-c]isoquinoline-1,3,7,12(2H,4H)-tetraones as new leads against Mycobacterium tuberculosis.

dialkyltetrahydrobenzo[g]pyrimido [4,5-c]isoquinolinetetraones were designed and synthesised. The compounds were submitted to a biological evaluation in which the activity against M.tb H37R v(lux) was observed, as well as the acute ...

In vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials

(these) activity(ies) will be explored using cell based transactivational assays also named “reporter gene assays. The MCF7 recombinant cells used here are genitically modified cells containing the firefly ...

Evaluation of a screening system for obesogenic compounds: screening of endocrine disrupting compounds and evaluation of the PPAR dependency of the effect.

adipogenesis model, and direct fluorescent measurement using Nile red lipid staining technique. In a first phase, we characterised the assay using the acknowledged obesogens rosiglitazone and tributyltin. Based ...

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