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Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.

achieved by whole-genome sequencing. In this chapter, we describe a robust, cost-effective, and widely applicable protocol for reconstructing (nearly) complete CaPV genomes directly from clinical samples or ...

A robust, cost-effective and widely applicable whole-genome sequencing protocol for capripoxviruses

Publication Type: Peer reviewed scientific article Authors: Elisabeth Mathijs; Andy Haegeman; Kris De Clercq; Steven Van Borm; Frank Vandenbussche Source: Journal of Virological Methods (2022) Health Topics:  Animal health Santé animale Diergezondheid Ful ...

Overview of diagnostic tools for Capripox virus infections.

with an advantageous cost-benefit balance. This review aims to look at existing and new virological and serological diagnostic tools used in the control against diseases caused by Capripox viruses. ...

Scalable chromatography-based purification of virus-like particle carrier for epitope based influenza A vaccine produced in Escherichia coli.

downstream processes. In particular, a rapid low cost purification process has been identified as a remaining key challenge in manufacturing process development. In the present study we set up a size-exclusion ...

Skin mucus of Cyprinus carpio inhibits cyprinid herpesvirus 3 binding to epidermal cells.

Publication Type: Peer reviewed scientific article Authors: Raj, Victor Stalin; Fournier, Guillaume; Rakus, Krzysztof; Ronsmans, Maygane; Ouyang, Ping; Michel, Benjamin; Delforges, Cédric; Costes ...

Diagnostic performance and application of two commercial cell viability assays in foot-and-mouth disease research.

measures (1.00, 0.98 and 0.99, respectively) and Cohen kappa agreement indices (0.97, 0.97 and 0.96, respectively) for detecting CPE in cell cultures. Due to its performance, cost effectiveness and ease of ...

Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

RT-qPCR failure due to poor sample quality, improper nucleic acid extraction and/or PCR inhibition. To increase the diagnostic capacity and reduce costs, it is recommended to use a multiplex strategy which ...

The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions.

Publication Type: Peer reviewed scientific article Authors: Michel, B; Leroy, B; V Stalin Raj; Lieffrig, F; Jan Mast; Wattiez, R; Vanderplasschen, A F; Costes, B Source: J Gen Virol, Volume 91, ...

Lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles.

agglomeration, high costs, and upscaling difficulties. Additionally, there exists a need for the development of a more eco-friendly production of nanosilver. In this study, Gram-positive and Gram-negative bacteria ...

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