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Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate.
H9N2 clones, selected on different cell types, named MDCK-and CEF-clone in regards to the cell culture used, were studied in vivo, revealing two different virulence phenotypes. Subsequently, the ...
RNA-based drug susceptibility testing of Mycobacterium tuberculosis
the knowledge on resistance mutations which is limited for especially 2nd-line and new drugs, (b) do not distinguish living from death cells, (c) ignore all intrinsic resistance mechanisms like efflux ...
Subcutaneous Granulomatous Inflammation due to Basidiobolomycosis: Case Reports of 3 Patients in Buruli Ulcer Endemic Areas in Benin.
widely available in endemic areas and with varying sensitivity. A combination of histopathological findings, namely, granulomatous inflammation with giant cells, septate hyphal fragments, and the ...
Endocrine activity of mycotoxins and mycotoxin mixtures.
Source: Food Chem Toxicol, Volume 96, p.107-16 (2016) Keywords: Breast Neoplasms Cells, Cultured Endocrine disruptors Female Genes, Reporter Humans Luciferases Mycotoxins Osteoblasts Poisons PPAR gamma ...
Pseudorabies virus isolates from domestic pigs and wild boars show no apparent in vitro differences in replication kinetics and sensitivity to interferon-induced antiviral status.
Animals Antiviral Agents Cells, Cultured Herpesvirus 1, Suid Interferons Sus scrofa Viral Plaque Assay Virus Replication Abstract: Pseudorabies virus is the causative agent of Aujeszky’s disease. Domestic ...
Growth of Stressed Strains of Four Non-O157 Shiga Toxin-Producing Escherichia coli Serogroups in Five Enrichment Broths.
Keywords: Culture Media Serogroup Shiga-Toxigenic Escherichia coli Abstract: The purpose of this study was to evaluate (i) the behavior of several strains of non- O157 Shiga toxin-producing Escherichia coli ...
Effect of exposure to stress conditions on propidium monoazide (PMA)-qPCR based Campylobacter enumeration in broiler carcass rinses
quantification by qPCR is unable to distinguish viable vs. dead cells in contrast to the culture-based ISO 10272-2 reference method. Propidium monoazide (PMA) has been used to overcome this disadvantage. ...
Comparison of sample types and analytical methods for the detection of highly campylobacter-colonized broiler flocks at different stages in the poultry meat production chain.
samples. PMA treatment before qPCR inhibits the signal from dead Campylobacter cells. Consequently, when samples are extensively stored and/or transported, qPCR is preferred to direct culture and PMA-qPCR. ...
Biosafety Recommendations on the Handling of Animal Cell Cultures
Publication Type: Scientific book or chapter Authors: Philippe Herman; Katia Pauwels; Al-Rubeai,Mohamed Source: Animal Cell Culture, Springer International Publishing, Volume 9, Issue 22, Number ...
Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis.
non-total extinction of the signal of dead cells sample in the v-qPCR assay. Indeed, the data strongly indicate that the remaining qPCR signal observed in non-culturable cells does not only depend on the ...