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Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different isolates. Genetic variation can however result in mismatches of primers and probes with their ...
Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.
Volume 7 (2017) Keywords: INFLUENZA non coding regions Abstract: For each influenza virus genome segment, the coding sequence is flanked by non-coding (NC) regions comprising shared, conserved sequences ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
identification of ESBL s and carbapenemases in Gram-negative bacilli. This assay had to be (i) superior to traditional (multiplex) PCR /sequencing-based tests in turn-around time, gene coverage and the ability to ...
In silico epitope analysis of unique and membrane associated proteins from Mycobacterium avium subsp. paratuberculosis for immunogenicity and vaccine evaluation.
and/or diagnosis. 110 protein sequences were selected from MAP K-10 genome database: 48 classified as putative enzymes involved in surface polysaccharide and lipopolysaccharide synthesis, as membrane ...