Search results - 10 results
Development of a Droplet Digital PCR to Monitor SARS-CoV-2 Omicron Variant BA.2 in Wastewater Samples
assay. Subsequently, the sensitivity and specificity of the T19I assay were successfully experimentally evaluated. Thanks to our specific method design, the positive signal from the mutant probe and ...
Sex differences in diabetic foot ulcer severity and outcome in Belgium
were used. Results The vast majority of patients included were male (72%). Ulcers in men were deeper, more frequently displaying probe to bone, and more frequently deeply infected. Twice as many men presented ...
Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution.
SARS-CoV-2 for the selection of the two PCR assays targeting two different regions in the genome, and to monitor the possible impact of virus evolution on the specificity of the primers and probes of the PCR ...
Particle size analysis of pristine food-grade titanium dioxide and E 171 in confectionery products: Interlaboratory testing of a SP-ICP-MS screening method and confirmation with transmission electron microscopy.
probe sonication was required. Repeatability and reproducibility were below 10% and 25% respectively for most measurands except for the lower (D0) and the upper (D100) bound of the particle size ...
Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants
Keersmaecker Source: Genes, Volume 12, Issue 4 (2021) Keywords: SARS-CoV-2; COVID-19; variants; detection; RT-qPCR; in silico specificity evaluation; bioinformatics tool; WGS data; mismatches; primers and probes ...
Residing in urban areas with higher green space is associated with lower mortality risk: A census-based cohort study with ten years of follow-up
used to probe into cause-specific mortality (non-accidental, respiratory, COPD, cardiovascular, ischemic heart disease (IHD), and cerebrovascular). Models were adjusted for several sociodemographic ...
Challenges to Differentiate Hepatitis C Genotype 1 and 6: Results from A Field-Study in Cambodia.
LightPower Taqman real-time PCR method (Viet A Corp.) and quality controlled using the Versant 2.0 line probe assay (Siemens Healthcare). Next, following interim quality control results, all 106 samples were ...
Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.
(consisting of a total of 30 primers and probe sets) used for SARS-CoV-2 detection and the impact of the virus’ genetic evolution on four of them. The exclusivity of these methods was also assessed using the ...
Development of a genoserotyping system for the identification of Salmonella serotypes
biochemical test. From this molecular markers’ selection, 4 MOL- PCR assays were developed, i.e. the molecular markers were recognized by probes through a ligation-amplification reaction (MOL- PCR), followed by ...
Physicochemical Characterisation of the E171 Food Additive
sample preparation protocols influencing particle dispersion (pH, probe sonication and centrifugation) were tested and optimized. In optimized conditions, representative TEM micrographs could be recorded, ...