Search results - 7 results
Particle size analysis of pristine food-grade titanium dioxide and E 171 in confectionery products: Interlaboratory testing of a SP-ICP-MS screening method and confirmation with transmission electron microscopy.
probe sonication was required. Repeatability and reproducibility were below 10% and 25% respectively for most measurands except for the lower (D0) and the upper (D100) bound of the particle size ...
Development of a genoserotyping system for the identification of Salmonella serotypes
biochemical test. From this molecular markers’ selection, 4 MOL- PCR assays were developed, i.e. the molecular markers were recognized by probes through a ligation-amplification reaction (MOL- PCR), followed by ...
A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.
biotinylated probes with the capture of those probes with streptavidin-coated paramagnetic beads, T. gondii DNA can selectively be “fished out” from a large volume of meat lysate. Still, several studies showed ...
Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.
(2017) Keywords: Air Pollution, Indoor DNA Probes environment Fungi Limit of Detection Nucleic Acid Hybridization Abstract: Considered as a public health problem, indoor fungal contamination is generally ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
probe targeting a conserved 16S rRNA region serving as a DNA extraction control. The assay was optimized using a collection of 48 reference strains and further validated using 105 foodborne ESBL-producing ...
Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.
p.790170 (2015) Keywords: DNA Primers DNA, Bacterial Humans Microspheres Multiplex Polymerase Chain Reaction Oligonucleotide Probes Salmonella typhimurium Abstract: With multiplex oligonucleotide ligation- ...
Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology
types of Luminex assays can be envisaged. Each assay has its advantages and limitations, some of which are related to the inherent design of the primers and probes used. Software that predicts melting ...