Search results - 106 results

Application of a cell-based protease assay for testing inhibitors of picornavirus 3C proteases.

Agents Cell Line Cysteine Endopeptidases Drug Evaluation, Preclinical Genes, Reporter Humans Luciferases, Firefly Picornaviridae Protease Inhibitors Viral Proteins Abstract: Proteolytical cleavage of the ...

Genotoxicity evaluation of nanosized titanium dioxide, synthetic amorphous silica and multi-walled carbon nanotubes in human lymphocytes.

properties of NM s, including size and morphology, might influence their toxicity and have impact on human health. The present work aimed to evaluate the genotoxicity of nanosized titanium dioxide (TiO2), ...

Effect of a DIVA vaccine with and without in-feed use of coated calcium-butyrate on transmission of Salmonella Typhimurium in pigs.

crucial, although additional control measures on-farm need to be considered. This study evaluated the effect of two potential control measures against the spread of Salmonella Typhimurium via a transmission ...

Development, validation and evaluation of added diagnostic value of a q(RT)-PCR for the detection of genotype A strains of small ruminant lentiviruses.

Publication Type: Peer reviewed scientific article Authors: Nick De Regge; Ann Brigitte Cay Source: J Virol Methods, Volume 194, Issue 1-2, p.250-7 (2013) Keywords: Animals Belgium Goat Diseases Goats Lentivirus Lentivirus Infections Molecular Diagnostic ...

Development and validation of three Capripoxvirus real-time PCRs for parallel testing.

different analytical and diagnostic parameters need to be evaluated to assure data quality. The implementation of parallel testing using multiple real-time PCR s with similar characteristics can improve ...

Establishing mono-eukaryotic Histomonas meleagridis cultures from in vivo infection contaminated with Tetratrichomonas gallinarum and Blastocystis spp.

were isolated and inoculated into culture media pre-incubated with caecal bacteria. After 48 h of incubation, presence of H. meleagridis in the cultures was confirmed through morphological evaluation ...

Effect of saliva stabilisers on detection of porcine reproductive and respiratory syndrome virus in oral fluid by quantitative reverse transcriptase real-time PCR.

Abstract: This study evaluated the effect of extraction-amplification methods, storage temperature and saliva stabilisers on detection of porcine reproductive and respiratory syndrome virus (PRRSV) RNA by ...

Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.

recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. ...

Trends in genotype frequency resulting from breeding for resistance to classical scrapie in Belgium (2006 ˜ 2011).

(n = 6,437), the ARQ haplotype diminished from 21.1% to 12.9%, and the VRQ haplotype decreased from 2.0% to 1.7%. The status of codon 141, a determinant for atypical scrapie, was also evaluated. Out of ...

Diagnosis of Schmallenberg virus infection in malformed lambs and calves and first indications for virus clearance in the fetus.

thoracic fluids of respectively 55 malformed lambs and calves were examined by a virus neutralization test (VNT) to evaluate the presence of neutralizing anti- SBV antibodies in these animals. Our results ...

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