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Analytical performance of several classical swine fever laboratory diagnostic techniques on live animals for detection of infection.
evaluated. The RT-nPCR detected the highest proportion of infected pigs (98.9%), whereas the Antigen ELISA gave the worst detection results (74.7%). Within the group of test positive animals, infection was ...
Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-gamma in response to mitogen and recall Newcastle disease viral antigen stimulation.
potential of a capture enzyme-linked immunosorbent assay (ELISA) specific for chicken interferon-gamma (ChIFN-gamma) has been evaluated as a tool to assess cell-mediated immunity (CMI) in the chicken. In ...
Evaluation of virulence of Mycoplasma hyopneumoniae field isolates.
factors, including differences in virulence between M. hyopneumoniae strains, may also be involved. The aim of this study was to evaluate the virulence of six M. hyopneumoniae field isolates and link it to ...
Real-time PCR for simultaneous detection and genotyping of bovine viral diarrhea virus.
isolates of both genotypes. The application to clinical diagnosis was evaluated on pooled blood samples by post run measurement of the FAM- and VIC-associated fluorescence. The 100% agreement with the ...
Isolation of a glycoprotein E-deleted bovine herpesvirus type 1 strain in the field.
Abstract: During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV-1) marker vaccines, a glycoprotein E (gE)-negative BHV-1 strain was isolated from the nasal ...
Molecular analysis of the capsid coding region of a virulent encephalomyocarditis virus isolate after serial cell passages and assessment of its virulence.
order to evaluate the genetic variability of the CCR of an highly virulent Belgian EMCV isolate after 210 passages in cell culture, this region has been molecularly characterised by RT- PCR and ...
An experimental infection to investigate the indirect transmission of classical swine fever virus by excretions of infected pigs.
evaluate the effect of factors like virus strain, interval, …, that may influence the outcome of the experiment. Health Topics: Animal health Category: A1 FOS Classification: 4.03 Veterinary science ...
Development of a capture ELISA for the detection of antibodies to enteropathogenic Escherichia coli (EPEC) in rabbit flocks using intimin-specific monoclonal antibodies.
reactors were obtained. The test characteristics improve with increasing prevalence. To evaluate the diagnostic potential of the cELISA, sera from 40 to 50 slaughter rabbits per flock from 25 rabbit flocks ...
Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs.
a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD- TOPO- TA expression vector permitted to evaluate the ...
Diagnosis of foot-and-mouth disease by RT-PCR: use of phylogenetic data to evaluate primers for the typing of viral RNA in clinical samples.
Publication Type: Peer reviewed scientific article Authors: Reid, S M; Ferris, N P; Hutchings, G H; Kris De Clercq; Newman, B J; Knowle, N J; Samuel, A R Source: Arch Virol, Volume 146, Issue 12, p.2421-34 (2001) Keywords: Animals DNA Primers Foot-and-Mou ...