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Vaccination of mice using the West Nile virus E-protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects mice against a lethal challenge.
E-specific IL-4 T-cell immune responses were detected by ELISPOT after protein boost and CD8 (+) specific IFN-γ expression was observed by flow cytometry. Challenge experiments using the heterologous ...
The Enrichment of Histomonas meleagridis and Its Pathogen-Specific Protein Analysis: A First Step to Shed Light on Its Virulence.
cells from a mono-eukaryotic culture copropagated with host cecal bacteria by flow cytometry. The enrichment of histomonads was confirmed through transmission electron microscopy and two-dimensional gel ...