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Metagenomic sequencing determines complete infectious bronchitis virus (avian Gammacoronavirus) vaccine strain genomes and associated viromes in chicken clinical samples.
identified both fixed and minor frequency variations in the tissue sample compared to its in ovo-passaged virus. Metagenomic methods allow the determination of complete coronavirus genomes from clinical ...
Comparative genomics of quinolone-resistant and susceptible Campylobacter jejuni of poultry origin from major poultry producing European countries (GENCAMP)
known which is the most accurate method for identifying the country of origin of the isolates. Allele frequency analysis of isolates under this study and a selection of previously published C. jejuni ...
Effect of residual doxycycline concentrations on resistance selection and transfer in porcine commensal escherichia coli.
significantly higher at 1 and 4 mg/L DOX compared to the blank control, but no significant difference between 1 and 4 mg/L was seen. Plasmid transfer frequencies were affected by 1 or 4 mg/L DOX in the medium for ...
Next-generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model.
next-generation sequencing resulting in an average depth of at least 700 × in each sample. Appropriate controls were sequenced to discriminate biologically relevant low-frequency variants from experimentally ...
Experimental Infection of Sheep at 45 and 60 Days of Gestation with Schmallenberg Virus Readily Led to Placental Colonization without Causing Congenital Malformations.
lambs. Time of inoculation (45 or 60 dg) had no impact on the placental colonization success rate but affected the frequency of detecting the virus in the offspring extraembryonic structures by the time of ...
A survey on biosecurity and management practices in selected Belgian cattle farms.
a combination of a linear scoring system, a categorical principal component analysis and a two-step cluster analysis to differentiate these farms based on their biosecurity levels and visit frequencies. Further ...
Schmallenberg virus circulation in culicoides in Belgium in 2012: field validation of a real time RT-PCR approach to assess virus replication and dissemination in midges.
a frequency analysis of the Ct values obtained for 31 SBV-S segment positive pools of Avaritia midges showed a clear bimodal distribution with peaks of Ct values between 21-24 and 33-36. This closely resembles ...
Genotoxicity evaluation of nanosized titanium dioxide, synthetic amorphous silica and multi-walled carbon nanotubes in human lymphocytes.
metabolic activation), we observed significant increases in the frequencies of micronucleated binucleated cells (MNBC s) for some TiO2 NM s and for two MWCNT s, although no clear dose-response relationships ...
Trends in genotype frequency resulting from breeding for resistance to classical scrapie in Belgium (2006 ˜ 2011).
implemented a breeding program that promotes selection of the ARR haplotype while reducing the incidence of VRQ. From 2006 to 2011 in Belgium, frequency for the ARR / ARR genotypes increased from 38.3% to 63.8% ...
An essential role for γ-herpesvirus latency-associated nuclear antigen homolog in an acute lymphoproliferative disease of cattle.
This hypothesis was further supported by the high frequencies of infected CD8 (+) T cells during MCF using immunodetection of ORF73 protein and single-cell RT- PCR approaches. Finally, the role of ...