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The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.

in relation to GC-content, predicted RNA secondary structure and sequence complementarity to the 3’ part of the tag sequence, that the tag sequence has the main contribution to the observed bias in ...

Deoxynivalenol loads in matched pair wheat samples in Belgium: comparison of ELISA VERATOX kit against liquid chromatography.

GC methods (with or without MS). However, networking of laboratories using this rapid method and laboratories with reference analytical methods should be encouraged. Health Topics:  Animal health ...

Prime-boost strategies combining DNA and inactivated vaccines confer high immunity and protection in cattle against bovine herpesvirus-1.

cellular arms of the immune response primed with Bovine herpesvirus-1 (BoHV-1) DNA vaccines encoding glycoprotein D (gD) or gC+gD. Calves vaccinated according to the DNA prime-inactivated boost also showed ...

Inhibition of bovine sperm-zona binding by bovine herpesvirus-1.

was treated with monoclonal antibodies against the viral glycoproteins gB, gC, gD and gH prior to insemination. Anti-gC completely prevented the inhibitory effect of BoHV-1 on sperm-zona binding, while ...

Arcobacter cibarius sp. nov., isolated from broiler carcasses.

hybridization with reference strains of the current four Arcobacter species revealed binding levels below 47 %. The G+C contents ranged between 26.8 and 27.3 mol%. Pairwise comparison of 16S rRNA gene sequences ...

Acinetobacter diversity in environmental samples assessed by 16S rRNA gene PCR-DGGE fingerprinting.

species. PCR using the Acinetobacter primer pair allowed detection of Acinetobacter in soil with a detection limit of 10(4) cells g(-1) soil, but attachment of the GC-clamp to the forward primer resulted in ...

Genetic immunisation of cattle against bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8.

induces higher neutralising antibody titres than vaccines encoding BoHV-1 gC. Additionally, we show that a DNA vaccine encoding a secreted form of gD induces a higher immune response than a vaccine encoding ...

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