Search results - 130 results

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

high-throughput sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

complying with the Minimum Performance Requirements for Analytical Methods of GMO Testing that is used as a standard by the GMO enforcement laboratories. The method’s applicability was also tested on 25 ...

Development, evaluation and optimization of next generation sequencing data analysis tools in support of a fast response for public health and food chain safety

Publication Type: Dissertation Authors: Assia Saltykova Source: Ghent University, Ghent, Belgium, p.308 (2022) Keywords: bioinformatics tools Database GMO Metagenomics NGS outbreak investigation ...

Plants with stacked genetically modified events: to assess or not to assess?

Number 73, p.70-3 (2014) Keywords: Genetically modified organism Safety assessment stacked GM event Abstract: The principles for the safety assessment of genetically modified (GM) organisms (GMO s) are ...

Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database

Publication Type: Peer reviewed scientific article Authors: Saltykova, Assia; Julien Van Braekel *; N. Papazova *; Marie-Alice Fraiture *; Deforce, Dieter; Vanneste, Kevin; Sigrid C.J. De Keersmaecker; Nancy Roosens * Source: Food Chemistry: Molecular Sci ...

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

real-time PCR GMM protease2 marker was also demonstrated on microbial fermentation products. To confirm its use by other GMO enforcement laboratories, the transferability of the in-house validated real-time ...

Food Chemistry, Function and AnalysisDNA Techniques to Verify Food Authenticity CHAPTER 8. GMO Detection and Identification Using Next-generation Sequencing

106 (2020) ISBN: 978-1-78801-178-5 Keywords: GMO Detection and Identification Using Next-generation Abstract: ext-generation sequencing (NGS) technologies are being increasingly evaluated for their ...

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

walking food and feed safety GMO GMO detection method method development nanopore sequencing Unauthorised GMO Abstract: In order to strengthen the current genetically modified organism (GMO) detection ...

Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Characterization detection DNA walking GMO identification qPCR Abstract: Background Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences ...

Monitoring of Laboratory-Acquired Infections

biosafety optimization. In this regard, blame-free reporting of LAI s is essential. A remarkable finding is that LAI s with genetically-modified organisms (GMO s) hardly seem to occur. This is likely due to ...

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