Search results - 14 results
Introduction of WGS in food microbiology: advantages and challenges
for the Safety of the Food Chain (FASFC) published the opinion 18-2021 regarding whole genome sequencing (WGS) for the detection of foodborne outbreaks and bacterial risk assessment. This opinion was ...
Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies
detection and for fast outbreak investigation that can be implemented in routine laboratories on samples prepared with the current standard practices. Health Topics: Food-borne illness Voedseltoxi-infecties ...
Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks
staphylococcal food poisoning (SFP) whole genome sequencing Abstract: Through staphylococcal enterotoxin (SE) production, Staphylococcus aureus is a common cause of food poisoning. Detection of staphylococcal food ...
Staphylococcal enterotoxin gene cluster: prediction of enterotoxin (SEG and SEI) production and of the source of food poisoning based on Saβ typing.
many people. Most of these outbreaks remain undiscovered as detection methods for enterotoxins are only available for a few enterotoxins, but not for the more recently discovered enterotoxin G (SEG) and ...
Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.
shotgun sequencing of food samples, could not only reduce the turnaround time of samples in an outbreak investigation, but also allow the detection of multi-species and multi-strain outbreaks. For ...
The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.
enabled an accurate cluster definition, which remained unsolved for the 2013 outbreak, partly due to scarce epidemiological linking with the suspect source. Moreover, it allowed detecting two and one ...
NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Strains Responsible for FoodBorne Outbreaks.
Hristo Daskalov; Michel-Yves Mistou; Jacques-Antoine Hennekinne Source: Front Microbiol, Volume 11 (2020) Keywords: bioinformatics tool; gene detection genomic staphylococcal enterotoxins genes variant ...
Use of next‐generation sequencing in microbial risk assessment
applications are not new in food microbiology with applications ranging from pathogen detection along the food chain, food epidemiology studies, whole genome analysis of food‐associated microorganisms up to ...
Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
Microbiology and Biotechnology, Volume 102, Issue 7, Number 3285 (2018) Keywords: Detection; Pathogenic E. coli; Real-time PCR; STEC; SYBR® Green; Validation Abstract: A detection and discrimination system for ...
A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods
to support EC legislation. As part of this mandated work programme, merging of ISO / TS 21872-1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ...