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Recombinant aequorin as a reporter for receptor-mediated changes of intracellular Ca2+-levels in Drosophila S2 cells4412
concentration-dependent bioluminescent responses in these S2- STKR-Aeq cells. The EC (50) value for the calcium effect detected by means of aequorin appeared to be nearly identical to the one that was measured by means of ...
Diagnosis of foot-and-mouth disease by RT-PCR: use of phylogenetic data to evaluate primers for the typing of viral RNA in clinical samples.
samples under investigation. The analyses showed that the design of primer sets for the detection of FMD virus serotypes O, A, Asia 1, SAT 1 and SAT 3 were generally satisfactory, as most virus isolates ...
A transcriptional luxAB reporter fusion responding to fluorene in Sphingomonas sp. LB126 and its initial characterisation for whole-cell bioreporter purposes.
use as a whole-cell biosensor in the perspective of quantifying fluorene in environmental samples. Activity of the reporter gene as a response to fluorene was detectable after 30 min and was optimal ...
A PCR test to identify bacillus subtilis and closely related species and its application to the monitoring of wastewater biotreatment.
applicable to single colonies and showed excellent specificity. In the mixed population context of wastewater analysis, direct detection of the target Bacillus species by PCR on either crude or purified DNA ...
Tracability in meat processing: The genetic markers212
p.15- 24 (2001) Keywords: 2012 a alleles Automation Belgium Class Cost de detection differences Field Genetic Genetic Markers Genome IS IT LEVEL Marker Markers Mass Mass Spectrometry Meat Mutation ON ...
Purified bovine WC1+ gamma delta T lymphocytes are activated by staphylococcal enterotoxins and toxic shock syndrome toxin-1 superantigens: proliferation response, TCR V gamma profile and cytokines expression.
family were induced. Genes encoding TCR Vgamma1 and TCR Vgamma2 family, but not TCR Vgamma5 were detected. The cells also expressed cytokine transcripts, namely, those of IL-12, IFN-gamma and TNF-alpha, ...
Morbillivirus in common seals stranded on the coasts of Belgium and northern France during summer 1998.
was observed in a few lymphocytes in the spleen and lymph nodes of three seals. Anti- CDV neutralising antibodies were detected in sera from six animals. Seven of the seals were positive by reverse ...
Descriptive epidemiology of a classical swine fever outbreak in the Limburg Province of Belgium in 1997.
virological findings indicated that the CSF-infected herds were detected in an early stage of the infection. The early detection of the infection together with a preventive stamping out procedure resulted in ...
p53 expression and apoptosis in melanomas of dogs and cats.
For all cases clinical follow-up was available. p53 expression was examined immunohistochemically using different monoclonal and polyclonal antibodies. Apoptosis was detected using the TUNEL technique. ...
Genotypic characterization of enteropathogenic Escherichia coli (EPEC) isolated in Belgium from dogs and cats41
pathogenesis of EPEC infection. The presence of these genetic elements was investigated in 14 dog and three cat EPEC isolates. A bfpA-related gene was detected in five of the 17 isolates in association with high ...