Search results - 40 results

Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products

for Analytical Methods of GMO Testing” of the European Network of GMO Laboratories. The validated real-time PCR TR method was also demonstrated to be applicable to commercial microbial fermentation ...

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) organisms ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

complying with the Minimum Performance Requirements for Analytical Methods of GMO Testing that is used as a standard by the GMO enforcement laboratories. The method’s applicability was also tested on 25 ...

Development, evaluation and optimization of next generation sequencing data analysis tools in support of a fast response for public health and food chain safety

Publication Type: Dissertation Authors: Assia Saltykova Source: Ghent University, Ghent, Belgium, p.308 (2022) Keywords: bioinformatics tools Database GMO Metagenomics NGS outbreak investigation ...

Plants with stacked genetically modified events: to assess or not to assess?

Number 73, p.70-3 (2014) Keywords: Genetically modified organism Safety assessment stacked GM event Abstract: The principles for the safety assessment of genetically modified (GM) organisms (GMO s) are ...

Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database

Publication Type: Peer reviewed scientific article Authors: Assia Saltykova; Julien Van Braekel; N. Papazova; Marie-Alice Fraiture; Deforce, Dieter; Kevin Vanneste; Sigrid C.J. De Keersmaecker; Nancy Roosens Source: Food Chemistry: Molecular Sciences, Vol ...

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

real-time PCR GMM protease2 marker was also demonstrated on microbial fermentation products. To confirm its use by other GMO enforcement laboratories, the transferability of the in-house validated real-time ...

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

Roosens Source: Sci Rep, Volume 8, Issue 1 (2018) Keywords: DNA walking food and feed safety GMO GMO detection method method development nanopore sequencing Unauthorised GMO Abstract: In order to strengthen ...

Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Characterization detection DNA walking GMO identification qPCR Abstract: Background Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences ...

How can we better detect unauthorized GMO in the food and feed chain

6, Number 517 (2017) Keywords: detection DNA walking Monitoring new workflow next-generation-sequencing qPCR unauthorized GMO Abstract: Current GMO detection systems have limited abilities to detect ...

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