Search results - 32 results

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

Sigrid C.J. De Keersmaecker; Kevin Vanneste; Nancy Roosens Source: Foods, Volume 12, Issue 3 (2023) Keywords: CRISPR/Cas9 detection food and feed chain Genetically modified organism high-throughput ...

Plants with stacked genetically modified events: to assess or not to assess?

Number 73, p.70-3 (2014) Keywords: Genetically modified organism Safety assessment stacked GM event Abstract: The principles for the safety assessment of genetically modified (GM) organisms (GMO s) are ...

Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus

to reconstruct from WGS data. This study aimed to improve the characterization, including the localization of AMR genes using short and long read WGS strategies. We used a genetically modified ...

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA ...

Model-Based Classification for Digital PCR: Your Umbrella for Rain.

of precision, both at individual partition level and at the level of the global concentration. We illustrate our method on genetically modified organism, inhibition, dynamic range, and mutation ...

How can we better detect unauthorized GMO in the food and feed chain

unauthorized genetically modified organisms (GMO s). Here, we propose a new workflow, based on next-generation sequencing (NGS) technology, to overcome this problem. In providing information about DNA sequences, ...

Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.

Volume 15, p.103 (2015) Keywords: Bacillus subtilis genetic engineering High-Throughput Nucleotide Sequencing Organisms, Genetically Modified Real-Time Polymerase Chain Reaction riboflavin Sensitivity and ...

Current and new approaches in GMO detection: challenges and solutions

International, Volume 2015, Issue 392872 (2015) Keywords: Current and New Approaches in GMO Detection: Challenges and Solutions Abstract: In many countries, genetically modified organisms (GMO) legislations have ...

Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing

number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...

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