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Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

the host depletion DNA extraction method. We then investigated the issue of the detection and characterization of genetically modified microorganisms (GMM s) in microbial fermentation products as a case ...

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

Sigrid C.J. De Keersmaecker; Kevin Vanneste; Nancy Roosens Source: Foods, Volume 12, Issue 3 (2023) Keywords: CRISPR/Cas9 detection food and feed chain Genetically modified organism high-throughput ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

flavorings Bacillus subtilis group Enzymes food and feed safety concerns real-time PCR detection Unauthorized genetically modified microorganisms Abstract: Most of the bacteria that are used to produce ...

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

Microbiology, Volume 354 (2021) Keywords: Antimicrobial resistance genes DNA walking anchored on pUB110 food and feed safety Genetically modified bacteria Protease real-time PCR detection Abstract: A PCR-based ...

A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products

Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Food Chemistry: Molecular Sciences, Volume 2 (2021) Keywords: AMR genetically modified microorganism identification long and short read sequencing ...

Food Chemistry, Function and AnalysisDNA Techniques to Verify Food Authenticity CHAPTER 8. GMO Detection and Identification Using Next-generation Sequencing

feasibility in supporting the current genetically modified organism (GMO) routine detection systems for unauthorized GMO. Depending upon the specific samples under investigation, two categories of NGS ...

Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products

resistance gene Food and feed microbial fermentation products Genetically modified microorganisms PCR-Based detection Abstract: Genetically modified microorganisms (GMM), harbouring commonly antimicrobial ...

Model-Based Classification for Digital PCR: Your Umbrella for Rain.

of precision, both at individual partition level and at the level of the global concentration. We illustrate our method on genetically modified organism, inhibition, dynamic range, and mutation ...

How can we better detect unauthorized GMO in the food and feed chain

unauthorized genetically modified organisms (GMO s). Here, we propose a new workflow, based on next-generation sequencing (NGS) technology, to overcome this problem. In providing information about DNA sequences, ...

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