Search results - 90 results
Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
Kathleen Marchal; Nancy Roosens; Sigrid C.J. De Keersmaecker; Kevin Vanneste Source: Microbial Genomics, Volume 7, Issue 3 (2021) Abstract: Whole genome sequencing (WGS) enables complete characterization of ...
Genomic epidemiology of emerging ESBL-producing Kentucky in Europe.
spread has occurred. Comparative genomics indicated that the gene is present on the bacterial chromosome, within the type VI secretion system region. The gene is integrated downstream of the gene, on ...
Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.
J (2020) Abstract: Conventional molecular tests for detecting complex (MTBC) drug resistance on clinical samples cover a limited set of mutations. Whole genome sequencing (WGS) typically requires culture. ...
Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination.
localized on plasmids can be easily transferred between bacterial isolates by horizontal gene transfer, thereby contributing to the spread of AMR. Next-generation sequencing (NGS) technologies are ideal for ...
A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.
chain reactions (qPCRs) are used in routine. In recent years, the use of whole genome sequencing (WGS) of bacterial isolates has proven its value to collect relevant information for strain ...
Use of a Whole Genome Sequencing-based approach for Mycobacterium tuberculosis surveillance in Europe in 2017-2019: an ECDC pilot study.
Dick van Soolingen; Marieke J van der Werf; Daniela Maria Cirillo; ECDC molecular surveillance project participants Source: Eur Respir J (2020) Abstract: Whole genome sequencing (WGS) can be used for ...
Phylogeography of Human and Animal Strains: Genetic Fingerprinting of Q Fever in Belgium.
reservoirs. To determine the relationship between animal and human strains, Multiple Locus Variable-number Tandem Repeat Analysis (MLVA) (n=146), Single-Nucleotide Polymorphism (SNP) (n=92) and Whole Genome ...
Development of a genoserotyping system for the identification of Salmonella serotypes
sequences of more than 230 000 Salmonella isolates), from the scientific literature and from genomic studies using publicly available and in-house produced Whole Genome Sequencing (WGS) data (achieving ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
Abstract: Whole genome sequencing (WGS) has proven to be the ultimate tool for bacterial isolate characterization and relatedness determination. However, standardized and harmonized workflows, e.g. for DNA ...
Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus
a major public health threat. Plasmids are able to transfer AMR genes among bacterial isolates. Whole genome sequencing (WGS) is a powerful tool to monitor AMR determinants. However, plasmids are difficult ...