Search results - 86 results
The impact of naturally-occurring, trans-placental bluetongue virus serotype-8 infection on reproductive performance in sheep.
in utero-infected lambs to the over-wintering of BTV appears limited. Health Topics: Animal health Category: A1 FOS Classification: 4.03 Veterinary science ...
A duplex real-time RT-PCR for the detection of bluetongue virus in bovine semen.
https://www.sciensano.be/en/biblio/a-duplex-real-time-rt-pcr-detection-bluetongue-virus-bovine-semen
the specificity was 100% (95% CI: 92-100%) and the limit of detection was-0.36 log(10) TCID (50) ml(-1) (95% CI:-0.53 to-0.18 log(10) TCID (50) ml(-1)) in BTV-8 spiked extended semen. The protocol was ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
internal/external control reactions were limited and the amount of Taq DNA polymerase was increased. A comparison of the singleplex versus triplex RT-qPCR indicated that the triplex RT-qPCR exhibits a higher ...
A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.
sensitivity, analytical specificity and intra- and interrun variability are described in detail. Special attention is given to the analytical sensitivity, which is determined by probit analysis. The limit of ...
Rapid detection of Eurasian and American H7 subtype influenza A viruses using a single TaqManMGB real-time RT-PCR.
avian influenza viruses (PanH7). The wide phylogenetic scope and analytical sensitivity and specificity were validated with the use of a panel of 56 diverse influenza A viruses. The detection limit was ...
Evaluation of rapid antigen detection kits for the diagnosis of highly pathogenic avian influenza H5N1 infection.
evaluation of the detection limits of the different tests established with serial dilution of HP H5N1 indicated that most of them have a detection limit of about 10(5) to 10(6) 50% tissue culture infectious ...
Development of specific enzyme-linked immunosorbent assays to evaluate the duck immune response after experimental infection with H5N1 and H7N1 low pathogenic avian influenza viruses.
mucosal surface may play an important role not only in protection but also in limitation of the primary replication at the portal of entry and shedding of the virus. With this aim, specific enzyme-linked ...
Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox.
this study, three suspended-growth oxygen-limited autotrophic nitrification-denitrification (OLAND) reactors with different inoculation and operation (mixing and aeration) conditions, designated reactors ...
Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.
effect on the analytical sensitivity was found for blood or foot epithelium. Approximately 1log(10) reduction in detection limit was noted for faecal and tongue epithelium samples, whereas a 3log(10) ...
Humoral, cell-mediated and mucosal immunity induced by oculo-nasal vaccination of one-day-old SPF and conventional layer chicks with two different live Newcastle disease vaccines.
chickens limited, if not completely abrogated, their immune responses to vaccination. This study increases our understanding of the protective immune response against Newcastle disease virus (NDV) and ...