Search results - 11 results

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

December 2019. COVID-19 cases are confirmed by the detection of SARS-CoV-2 RNA in biological samples by RT-qPCR. However, limited numbers of SARS-CoV-2 genomes were available when the first RT-qPCR methods ...

Evaluation of a TEM based Approach for Size Measurement of Particulate (Nano)materials

of the limit of detection, working range, selectivity, precision, trueness, robustness, and ruggedness. An uncertainty that was associated to intermediate precision in the range of 1–7% and an expanded ...

Development and validation of a quantitative UHPLC-MS/MS method for selected brominated flame retardants in food.

acidified silica (44%, w/w). The limits of quantification of the method varied widely for the types of food matrices and the different classes of BFR s from 4 pg g(-1) wet weight (ww) to 8 ng g(-1) ww. For ...

Scientific opinion on Malachite green in food

95% confidence limit for a benchmark response of 10% extra risk (BMDL10) of 13 mg/kg bw per day for hepatocellular adenomas and carcinomas was selected as reference point for neoplastic effects. For ...

Scientific Statement on Presence of microplastics and nanoplastics in food, with particular focus on seafood

nanoplastics as contaminants in food. Methods are available for identification and quantification of microplastics in food, including seafood. Occurrence data are limited. In contrast to microplastics no methods ...

A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay

(100 %), PCR efficiency (95.5 %), dynamic range and repeatability of this qPCR assay were subsequently evaluated. The limit of detection for this qPCR assay was determined to be 1 copy of genomic DNA of E. ...

Selenium content of Belgian cultivated soils and its uptake by field crops and vegetables.

accumulation is still limited at low soil Se concentrations. In loamy soils, weak correlations were found between the soil Se concentration and its concentration in wheat and potato. The uptake of Se increased ...

Scientific opinion on the risk for public and animal health related to the presence of sterigmatocystin in food and feed

Dietary exposure Dna effect effects European European Commission exposure exposure assessment fat feed food Food Chain Food Safety health Human human health IS Kidney Less Limit of Detection Liver Lung ...

Scientific opinion on emerging and novel brominated flame retardants (BFRs) in food

very limited information on occurrence, exposure and toxicity, the CONTAM Panel could not perform a risk characterisation for any of the BFR s considered. Instead, an attempt was made to identify those ...

Biosafety of molecular farming in genetically modified plants

some important issues linked to the assessment of environmental and health risks, and comment in more detail general management strategies that could be applied to limit potential environmental and human ...

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