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Search results - 18 results

Assessing the endocrine activity of PAHs using the DRE-, ERE- and PPARγ CALUX bioassays

was to determine the endocrine activity of 9 PAH s using three different CALUX bioassays: DRE- CALUX, ERE- CALUX and PPAR γ CALUX. Materials and methods Three CALUX (chemically activated luciferase gene ...

Endocrine activity of mycotoxins and mycotoxin mixtures.

Source: Food Chem Toxicol, Volume 96, p.107-16 (2016) Keywords: Breast Neoplasms Cells, Cultured Endocrine disruptors Female Genes, Reporter Humans Luciferases Mycotoxins Osteoblasts Poisons PPAR gamma ...

Biological activity conferred by endocrine disrupting chemicals in hospital effluent and river water from the Brussels Region, Belgium

routinely monitored in  targeted chemo-analytical methods and can detect them at low amounts (pg/L EEQ, Estrogen EQ uivalence).  The bioassay used in this study is the CALUX (Chemically activated luciferase ...

Genotoxicity testing of 3,4,5-trimethylfuran-2(5H)-one, a compound from plant-derived smoke with germination inhibitory activity.

Environ Mutagen, Volume 778, p.1-5 (2015) Keywords: 4-Nitroquinoline-1-oxide Benzopyrenes Cell Line, Tumor Cell Survival Furans Genes, Reporter Germination Hepatocytes Humans Luciferases Mutagenicity Tests ...

Application of a cell-based protease assay for testing inhibitors of picornavirus 3C proteases.

Cysteine Endopeptidases Drug Evaluation, Preclinical Genes, Reporter Humans Luciferases, Firefly Picornaviridae Protease Inhibitors Viral Proteins Abstract: Proteolytical cleavage of the picornaviral ...

In vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials

luciferase gene, as a reporter gene, and a DNA responsive element specific to the human oestrogen receptor. The biological activity of a chemical compound is monitored by the measurement of light emitted by ...

Feeding Cyprinus carpio with infectious materials mediates cyprinid herpesvirus 3 entry through infection of pharyngeal periodontal mucosa.

CyHV-3 in carp using bioluminescence imaging and a CyHV-3 recombinant strain expressing luciferase (LUC). We demonstrated that the skin is the major portal of entry after inoculation of carp by immersion ...

Intranasal DNA vaccination induces potent mucosal and systemic immune responses and cross-protective immunity against influenza viruses.

track following intranasal administration of luciferase-coding DNA. Using PEI formulation, intranasal vaccination with DNA-encoding hemagglutinin (HA) from influenza A H5N1 or (H1N1)2009 viruses induced ...

PCDD/Fs and dioxin-like PCBs in home-produced eggs from Belgium: levels, contamination sources and health risks.

Polychlorinated Biphenyls Risk Assessment Seasons Soil Abstract: This paper discusses the dioxin TEQ levels as determined by the chemically activated luciferase gene expression assay (CALUX) and by HRGC- HRMS in ...

The major portal of entry of koi herpesvirus in Cyprinus carpio is the skin.

using bioluminescence imaging. Taking advantage of the recent cloning of the KHV genome as a bacterial artificial chromosome (BAC), we produced a recombinant plasmid encoding a firefly luciferase (LUC) ...

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