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Application of a cell-based protease assay for testing inhibitors of picornavirus 3C proteases.
Agents Cell Line Cysteine Endopeptidases Drug Evaluation, Preclinical Genes, Reporter Humans Luciferases, Firefly Picornaviridae Protease Inhibitors Viral Proteins Abstract: Proteolytical cleavage of the ...
In vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
luciferase gene, as a reporter gene, and a DNA responsive element specific to the human oestrogen receptor. The biological activity of a chemical compound is monitored by the measurement of light emitted by ...
Feeding Cyprinus carpio with infectious materials mediates cyprinid herpesvirus 3 entry through infection of pharyngeal periodontal mucosa.
CyHV-3 in carp using bioluminescence imaging and a CyHV-3 recombinant strain expressing luciferase (LUC). We demonstrated that the skin is the major portal of entry after inoculation of carp by immersion ...
Intranasal DNA vaccination induces potent mucosal and systemic immune responses and cross-protective immunity against influenza viruses.
track following intranasal administration of luciferase-coding DNA. Using PEI formulation, intranasal vaccination with DNA-encoding hemagglutinin (HA) from influenza A H5N1 or (H1N1)2009 viruses induced ...
PCDD/Fs and dioxin-like PCBs in home-produced eggs from Belgium: levels, contamination sources and health risks.
Polychlorinated Biphenyls Risk Assessment Seasons Soil Abstract: This paper discusses the dioxin TEQ levels as determined by the chemically activated luciferase gene expression assay (CALUX) and by HRGC- HRMS in ...
The major portal of entry of koi herpesvirus in Cyprinus carpio is the skin.
using bioluminescence imaging. Taking advantage of the recent cloning of the KHV genome as a bacterial artificial chromosome (BAC), we produced a recombinant plasmid encoding a firefly luciferase (LUC) ...
Development of luminescent Mycobacterium avium subsp. paratuberculosis for rapid screening of vaccine candidates in mice37076
Luciferases Luminescent Measurements M mice MODEL Mycobacterium Mycobacterium avium subsp.paratuberculosis Paratuberculosis Plasmids Print Research Research Support SB- IM SCREENING Species TESTING ...
TEQ-value determinations of animal feed; emphasis on the CALUX bioassay validation
method is laborious and very expensive, therefore cheap and rapid bioassays have been developed. The chemical-activated luciferase bioassay (CALUX) bioassay uses a recombinant cell line, which responds to ...
Importance of REP values when comparing the CALUX bioassay results with chemoanalyses results Example with spiked vegetable oils.
Talanta, Volume 63, Issue 5, p.1255-9 (2004) Abstract: Differences between chemical activated luciferase gene expression (CALUX) bioassay and chemoanalyses results are observed. This paper shows that ...
Isoforms of cyclic AMP response element binding proteins in Drosophila S2 cells4408
embryonic insect cell line, i.e., Drosophila Schneider 2 (S2) cells. In addition, we have studied the possible effect of cellular cAMP- and Ca2+ increases on the expression of a luciferase reporter in cells ...