Search results - 10 results

Development of a multiplex-based immunoassay for the characterization of diphtheria, tetanus and acellular pertussis antigens in human combined DTaP vaccines.

focused on the development of a Luminex-based multiplex assay to monitor the consistency of antigen quantity and quality throughout the production process of DT aP vaccines from two human vaccine ...

Development of a multiplex immunoassay for simultaneous detection of antigens in DTaP vaccines

that could be implemented for routine potency assessment of vaccines. Our work focused on the development of a Luminex-based multiplex assay (four to six-plex assay depending on the number of pertussis ...

A molecular assay for rapidly distinguishing the AviPro SALMONELLA VAC T vaccine strain from wild-type field isolates

Volume 184 (2021) Keywords: Luminex-based multiplex test Molecular differentiation Salmonella typhimurium Vac T vaccin Abstract: Rapid differentiation of the AviPro Salmonella VAC T strain from ...

Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay.

using a Luminex-bead based assay and comparison to a virus neutralization test. While we show that neutralizing antibody levels are significantly lower in mild than in severe cases, we demonstrate that ...

Development of a genoserotyping system for the identification of Salmonella serotypes

detection of molecular markers or on the analysis of sequencing data, are described in the scientific literature. Based on a critical review of some of these techniques, the Multiplex Oligonucleotide ...

Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

misidentified E. coli samples at the National Reference Centre (NRC). In this paper, we describe two novel tools to enhance Shigella surveillance. First, we developed a low-cost Luminex-based multiplex assay ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

phenotypic Drug Susceptibility Testing (DST) of M. tuberculosis (Mtb), multiple DNA-based methods were successfully introduced over the last years. Although these are fast and sensitive, they (a) are based on ...

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

dead and uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this ...

Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.

identification of ESBL s and carbapenemases in Gram-negative bacilli. This assay had to be (i) superior to traditional (multiplex) PCR /sequencing-based tests in turn-around time, gene coverage and the ability to ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

is incubated in absence of the drug. Multiplex mRNA quantification is performed directly on crude cell lysates using a combination of the bead-based MagPixTM (Luminex) and QuantigeneTM Plex (Thermo ...

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