Search results - 18 results
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
combined in a four-plex assay that was thoroughly validated in-house. The SCREENED tool was used to evaluate the sequence coverage of the method. Results The full validation approach showed that the new ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.
the method. RESULTS: The full validation approach showed that the new four-plex method allows specific and sensitive identification and discrimination of Zika and chikungunya in routine samples. The combination ...
Validation of a Bioinformatics Workflow for Routine Analysis of Whole-Genome Sequencing Data and Related Challenges for Pathogen Typing in a European National Reference Center: as a Proof-of-Concept.
employed methods are “fit-for-purpose” and provide high-quality results. A harmonized framework with guidelines for the validation of WGS workflows does currently, however, not exist yet, despite several ...
Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java.
in-house, was developed in this study, to be applied to Salmonella isolates. This method was validated with the analysis of 178 S. Paratyphi B (D-tartrate fermenting and non-fermenting) and other serotypes ...
A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods
well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology ...
Fusarium, clinical relevance and mass spectrometry methods for identification, using strains of the Belgian fungal culture collection BCCM/IHEM
isolated from patients, were re-identified by multilocus DNA marker sequencing and phylogeny. The remaining 289 validated strains, comprising 40 different species, were of great value: (i) Analysis of the ...
Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015.
method was limited. The raw fragment length data were consistent throughout, and the inter-laboratory validation helped to standardise the conversion of raw data to repeat numbers with at least two ...
Antimicrobial resistance surveillance in Escherichia coli by using normalized resistance interpretation.
not susceptible. CONCLUSIONS: NRI was shown to be a valid method to define the WT population for disk diffusion outcomes, provided a normal distribution of the susceptible bacterial species population is present. ...
A case of acute intoxication due to combined use of fentanyl and 3,4-dichloro-N-[2-(dimethylamino)cyclohexyl]-N-methylbenzamide (U-47700).
reaction monitoring mode. The method validation was based on the Scientific Working Group of Forensic Toxicology document ‘Standard Practices of Method Validation in Forensic Toxicology’. In blood and urine ...
Validation of a Sampling Method to Collect Exposure Data for Central-Line-Associated Bloodstream Infections.
and 2012. SETTING AND PARTICIPANTS: CLD data were reported by 56 ICU s in 39 hospitals during 364 trimesters. METHODS: We compared estimated CLD data obtained from weekly and monthly sampling schemes with the ...