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Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution.

SARS-CoV-2 for the selection of the two PCR assays targeting two different regions in the genome, and to monitor the possible impact of virus evolution on the specificity of the primers and probes of the PCR ...

A molecular assay for rapidly distinguishing the AviPro SALMONELLA VAC T vaccine strain from wild-type field isolates

Volume 184 (2021) Keywords: Luminex-based multiplex test Molecular differentiation Salmonella typhimurium Vac T vaccin Abstract: Rapid differentiation of the AviPro Salmonella VAC T strain from ...

A multiplex oligonucleotide ligation-PCR method for the genoserotyping of common Salmonella using a liquid bead suspension assay

Publication Type: Peer reviewed scientific article Authors: Mathieu Gand; Wesley Mattheus; Roosens, Nancy H.C.; Dierick, Katelijne; Marchal, Kathleen; De Keersmaecker, Sigrid C.J.; Sophie Bertrand Source: Food Microbiology, Volume 87 (2020) Health Topics: ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

Myrielle; Rousset, Dominique; Van Gucht, Steven; Roosens, Nancy Source: International Journal of Infectious Diseases, Volume 92 (2020) Keywords: Chikungunya virus discrimination identification multiplex ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

a multiplex reverse transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, ...

Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

Luminex-based multiplex assay combining five genetic markers for species identification with 11 markers for serotype prediction for S. sonnei and S. flexneri isolates. Using a test panel of 254 clinical samples, ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

format. In short, 200 µl of a positively flagged MGIT culture is spiked with a specific concentration of a drug, while a control sample is incubated in absence of the drug. Multiplex mRNA quantification is ...

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

Volume 1616, p.39-69 (2017) Abstract: A multiplex oligonucleotide ligation- PCR (MOL- PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of ...

Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health

issues.  The multiplexing issue was overcome through a Luminex xMAP® assay, developed for the simultaneous detection of the 10 most frequently in indoor air found fungi. All the species identified with the ...

Proof-of-concept study of a new LC-ESI-MS/MS-based assay to identify Aspergillus spp. in artificially mixed samples using species/genus-specific proteotypic peptides

developed assay which can resolve most of these MALDI- TOF MS limitations. This assay is based upon multiplex liquid chromatography electrospray ionization tandem mass spectrometry (LC- ESI- MS / MS) for the ...

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