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Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.
misidentified E. coli samples at the National Reference Centre (NRC). In this paper, we describe two novel tools to enhance Shigella surveillance. First, we developed a low-cost Luminex-based multiplex assay ...
RNA-based drug susceptibility testing of Mycobacterium tuberculosis
format. In short, 200 µl of a positively flagged MGIT culture is spiked with a specific concentration of a drug, while a control sample is incubated in absence of the drug. Multiplex mRNA quantification is ...
Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).
Biol, Volume 1616, p.39-69 (2017) Abstract: A multiplex oligonucleotide ligation- PCR (MOL- PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for ...
Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
issues. The multiplexing issue was overcome through a Luminex xMAP® assay, developed for the simultaneous detection of the 10 most frequently in indoor air found fungi. All the species identified with the ...
Proof-of-concept study of a new LC-ESI-MS/MS-based assay to identify Aspergillus spp. in artificially mixed samples using species/genus-specific proteotypic peptides
developed assay which can resolve most of these MALDI- TOF MS limitations. This assay is based upon multiplex liquid chromatography electrospray ionization tandem mass spectrometry (LC- ESI- MS / MS) for the ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
identification of ESBL s and carbapenemases in Gram-negative bacilli. This assay had to be (i) superior to traditional (multiplex) PCR /sequencing-based tests in turn-around time, gene coverage and the ability to ...
Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR)
subtyping Multiplex assay ON pathogen PCR PRODUCTS protocol S Salmonella Salmonella enterica Salmonella typhimurium Subtyping Technique Type Viruses Abstract: A multiplex oligonucleotide ligation- PCR (MOL- ...
Development of xMAP and xTAG Luminex assays for public health surveillance
Publication Type: Scientific poster, presentation or proceeding Authors: Sigrid C.J. De Keersmaecker Source: xMAP® CONNECT 2016- Sharing Multiplex Solutions, NA, Issue Luminex Coorporation, NA ...
Optimized MOL-PCR for Characterization of Microbial Pathogens
different types of markers, like unique sequences, single-nucleotide polymorphisms (SNP s) and indels, can be simultaneously analyzed in one assay. This assay consists of a multiplex ligation for detection of ...
A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium
foodborne infections. In an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation- PCR (MOL- PCR) assay was developed which combines ...