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Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants

Source: Genes, Volume 12, Issue 4 (2021) Keywords: SARS-CoV-2; COVID-19; variants; detection; RT-qPCR; in silico specificity evaluation; bioinformatics tool; WGS data; mismatches; primers and probes ...

Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.

multiplex oligonucleotide ligation- PCR procedure was used, with read-out on a Luminex MAGPIX (®) platform. We designed 46 xTAG(®)-compatible probes targeting β-lactamase alleles and allele variants, and one ...

Optimized MOL-PCR for Characterization of Microbial Pathogens

Oligonucleotide Probes Phycoerythrin polymerase chain reaction Polymorphism, Single Nucleotide Salmonella typhimurium Streptavidin Temperature Abstract: Characterization of microbial pathogens is necessary for ...

Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.

(2015) Keywords: DNA Primers DNA, Bacterial Humans Microspheres Multiplex Polymerase Chain Reaction Oligonucleotide Probes Salmonella typhimurium Abstract: With multiplex oligonucleotide ligation- PCR ...

Clinical evaluation of a GP5+/6+-based luminex assay having full high-risk human papillomavirus genotyping capability and an internal control.

Oligonucleotide Probes Papillomaviridae Papillomavirus Infections Reference Standards Sensitivity and Specificity Young adult Abstract: The LMNX genotyping kit HPV GP (LMNX) is based on the clinically validated GP5 ...

Characterization of pathogenic and resistant genome repertoire reveals two clonal lines in Salmonella enterica subsp. enterica serovar Paratyphi B (+)-tartrate positive.

Burkhard Source: Foodborne Pathog Dis, Volume 6, Issue 4, p.431-43 (2009) Keywords: Animals Anti-Bacterial Agents Bacterial Typing Techniques Belgium Colony Count, Microbial DNA Probes DNA, Bacterial ...

Identification of endomorphin-1 and endomorphin-2 binding sites in human mu-opioid receptor by antisense oligonucleotide strategy4389

Measurements medical metabolism mRNA observed Oligonucleotides Oligonucleotides,Antisense Oligopeptides pain protein Receptor receptors Receptors,Opioid,mu Research Research Support response Responses Reverse ...

Identification of the aadB gene coding for the aminoglycoside-2"-O-nucleotidyltransferase, ANT(2"), by means of the polymerase chain reaction36645

Nucleotidyltransferases Oligonucleotides ON PCR polymerase chain reaction Print SB- IM SENSITIVITY specific specificity strain Technique Test Abstract: The polymerase chain reaction (PCR) was used to identify the gene ...

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