Search results - 134 results
Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.
commercial vaccine batches in less than a week. Taking advantage of the genetic similarity of CaPVs, a set of pan-CaPVs long-range PCR s was developed that covers the entire genome with only a limited number ...
RNA sequencing of avian paramyxovirus (Paramyxoviridae, Avulavirinae) isolates from wild mallards in Belgium, 2021: complete genomes and coinfections.
influenza virus (AIV) coinfection in all three virus isolates, confirming weak-positive AIV realtime RT- PCR results from the original sample material. In one AOAV-1 isolate, partial sequences covering all ...
Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains.
difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed and validated a new duplex real-time PCR capable of ...
Outbreaks of Foot-and-Mouth Disease in Burundi, East Africa, in 2016, Caused by Different Serotypes.
six provinces using rRT- PCR. FMDV was isolated from samples from three provinces: in Cibitoke province, serotypes A and SAT2 were isolated, while in Mwaro and Rutana provinces, only serotype SAT2 was ...
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison.
Véronique Duquesne; Severine Matthijs Source: Insects, Volume 13, Issue 1 (2021) Keywords: Aethina tumida Diagnosis honey bee inter-laboratory comparison Morphology Real-time PCR Small Hive Beetle Abstract: ...
Metagenomic sequencing determines complete infectious bronchitis virus (avian Gammacoronavirus) vaccine strain genomes and associated viromes in chicken clinical samples.
segment B closely related to D3976 /1 from Germany, 2017) and avian orthoreovirus, while three avian orthoavulavirus 1 reads confirmed prior real-time RT- PCR result. IBV sequence variation analysis ...
Detection of Clinical and Subclinical Lumpy Skin Disease Using Ear Notch Testing and Skin Biopsies.
Microorganisms, Volume 9, Issue 10 (2021) Abstract: Lumpy skin disease (LSD) diagnosis is primarily based on clinical surveillance complemented by PCR of lesion crusts or nodule biopsies. Since LSD can be ...
Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador
Silvia Pachacama; Jorge Espinoza; Hipatia Delgado; Maritza Barrera Source: Pathogens, Volume 10, Issue 11 (2021) Keywords: Bluetongue BTV Cattle competitive ELISA Ecuador Manabí PCR Abstract: Bluetongue ...
Retrospective survey of unauthorized genetically modified bacteria harbouring antimicrobial resistance genes in feed additive vitamin B2 commercialized in Belgium: Challenges and solutions
feed additive vitamin B2 products. To this end, two real-time PCR markers specific to this unauthorized genetically modified microorganism (GMM), named UGMV it- B2 and 558, were used. In the present ...
Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From Produced and Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer.
protocols used was successful in removing the viral genome completely from the produced and derived embryos as was demonstrated by real-time PCR. Moreover, BTV-8 virus was transmitted to recipient cows after ...