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Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products

resistance gene Food and feed microbial fermentation products Genetically modified microorganisms PCR-Based detection Abstract: Genetically modified microorganisms (GMM), harbouring commonly antimicrobial ...

Increased surface area of halloysite nanotubes due to surface modification predicts lung inflammation and acute phase response after pulmonary exposure in mice

inflammation. The pulmonary and hepatic acute phase responses were assessed by Serumamyloida mRNA levels in lung and liver tissue by real-time quantitative PCR. Pulmonary and systemic genotoxicity were analyzed ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

of two targets per virus allowing almost 100% coverage of about 500 genomes was shown for the first time. CONCLUSION: PCR being a reliable user-friendly technique, applicable in remote areas, such ...

Exploiting the Advantages of Molecular Tools for the Monitoring of Fungal Indoor Air Contamination: First Detection of in Indoor Air of Air-Conditioned Offices.

real-time PCR and massive parallel sequencing. The latter also allowed obtaining a broader view on the fungal diversity in the tested samples. Similar approaches were applied on water samples collected from ...

Imported malaria and artemisinin-based combination therapy failure in travellers returning to Belgium: A retrospective study.

polymorphisms in drug resistance-associated genes pfk13, pfcrt, pfmdr1, pfpm2, pfap2mu and pfubp1 were determined by sequencing or quantitative PCR. RESULTS: Annual malaria cases steadily increased in the last ...

Piacentinu Ennese PDO cheese as reservoir of promising probiotic bacteria.

in order to select promising probiotic strains to be further used in humans. One hundred and sixty-nine lactic acid bacteria (LAB) were isolated from 90 days ripened cheeses and identified by Rep- PCR ...

Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From Produced and Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer.

protocols used was successful in removing the viral genome completely from the produced and derived embryos as was demonstrated by real-time PCR. Moreover, BTV-8 virus was transmitted to recipient cows after ...

Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java.

biochemical tests. Therefore, a quadruplex real-time PCR method, based on the allelic discrimination of molecular markers selected from the scientific literature and from whole genome sequencing data produced ...

Strong increase of true and false positive mycobacterial cultures sent to the National Reference Centre in Belgium, 2007 to 2016.

by PCR in the NRC). We reviewed mycobacterial species identified and assessed trends over time of proportions of nontuberculous mycobacteria (NTM) vs complex (MTB c), and false positive cultures vs ...

Seroprevalence of border disease virus and other pestiviruses in sheep in Algeria and associated risk factors.

6 and 24 months of age (n = 576) and from lambs younger than 6 months (n = 113). All samples were tested by RT- PCR as well as by Ag- ELISA, to detect Persistently Infected (PI) animals. Serum samples ...

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