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Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.

tests, the limit of detection was 100-1000 genome copies for fixed resistance mutations. Deeplex Myc- TB captured in silico 97.1-99.3% of resistance phenotypes correctly predicted by WGS from 3651 MTBC ...

The African swine fever virus isolate Belgium 2018/1 shows high virulence in European wild boar.

Thus, the genotype II ASF virus isolate ‘Belgium 2018/1’ showed a highly virulent phenotype in European wild boar similar to parental viruses like Armenia 2007 and other previously characterized ASFV ...

Species Distinction in the Trichophyton rubrum Complex.

with a worldwide distribution. The members of the complex usually have distinct phenotypes in culture and cause different clinical symptoms, despite high genome similarity. In order to better delimit the ...

Antibiotic resistance prediction for Mycobacterium tuberculosis from genome sequence data with Mykrobe.

(WGS) data. Performance was insufficient to support the use of WGS as an alternative to conventional phenotype-based DST, due to mutation catalogue limitations.  Here we present a new tool,, which ...

Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

tools will be implemented in the workflow of the NRC, and will play a major role in the shift from phenotypic to genotyping-based surveillance of shigellosis in Belgium. Manuscript versions:  DOI:  ...

Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate.

H9N2 clones, selected on different cell types, named MDCK-and CEF-clone in regards to the cell culture used, were studied in vivo, revealing two different virulence phenotypes. Subsequently, the ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

phenotypic Drug Susceptibility Testing (DST) of M. tuberculosis (Mtb), multiple DNA-based methods were successfully introduced over the last years. Although these are fast and sensitive, they (a) are based on ...

Molecular epidemiology and antimicrobial resistance mechanisms of Campylobacter coli from diarrhoeal patients and broiler carcasses in Belgium.

in Belgium, by studying the genotypic diversity and antimicrobial resistance phenotypes and resistance mechanisms of 59 C. coli isolates. Isolates from broiler carcasses and human cases were genotyped ...

Prediction of Susceptibility to First-Line Tuberculosis Drugs by DNA Sequencing

accurately predict profiles of susceptibility to first-line antituberculosis drugs has not been clear. METHODS: We obtained whole-genome sequences and associated phenotypes of resistance or susceptibility to ...

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