Search results - 16 results

Phylogeography of Human and Animal Coxiella burnetii Strains: Genetic Fingerprinting of Q Fever in Belgium

Variable-number Tandem Repeat Analysis (MLVA) (n=146), Single-Nucleotide Polymorphism (SNP) (n=92) and Whole Genome Sequencing (WGS) (n=4) methods were used to characterize samples/strains during 2009-2019. Three ...

Phylogeography of Human and Animal Coxiella burnetii Strains: Genetic Fingerprinting of Q Fever in Belgium

Variable-number Tandem Repeat Analysis (MLVA) (n=146), Single-Nucleotide Polymorphism (SNP) (n=92) and Whole Genome Sequencing (WGS) (n=4) methods were used to characterize samples/strains during 2009-2019. Three ...

A bioinformatics WGS workflow for clinical complex isolate analysis, validated using a reference collection extensively characterized with conventional methods and approaches.

including (sub)species confirmation and identification (16S, / RD,, Single Nucleotide Polymorphism (SNP)-based antimicrobial resistance (AMR) prediction, and pathogen typing (spoligotyping, SNP barcoding, and ...

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

Source: Int J Mol Sci, Volume 21, Issue 15 (2020) Keywords: Betacoronavirus Coronavirus Infections Databases, Genetic Genome, Viral Humans Open Reading Frames Pandemics Pneumonia, Viral Polymorphism, Single ...

Use of a Whole Genome Sequencing-based approach for Mycobacterium tuberculosis surveillance in Europe in 2017-2019: an ECDC pilot study.

multilocus sequence typing (cgMLST), and single nucleotide polymorphism (SNP)-based calculation of distances on all WGS data that fulfilled minimum quality criteria to ensure data comparability.From 25 ...

Seasonal Genetic Drift of Human Influenza A Virus Quasispecies Revealed by Deep Sequencing

Mutation frequencies and single nucleotide polymorphisms were used for comparisons to address the level of natural intrinsic heterogeneity of influenza A viruses. Clear differences in single nucleotide ...

Data analysis associated with the use of whole genome sequencing for subtyping of pathogenic microorganisms, Salmonella Typhimurium as a case study

of a link between the origin of an infection and the human isolate in case of outbreak situations.Here, we have explored the possibilities offered by single nucleotide polymorphism (SNP)-based approach ...

Optimized MOL-PCR for Characterization of Microbial Pathogens

Oligonucleotide Probes Phycoerythrin polymerase chain reaction Polymorphism, Single Nucleotide Salmonella typhimurium Streptavidin Temperature Abstract: Characterization of microbial pathogens is necessary for ...

A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium

genomic island 1 (SGI1), allantoinase gene allB, MLVA locus STTR10, antibiotic resistance genes, single nucleotide polymorphisms (SNP s) and phase 2 flagellar gene fljB. The in vitro stability of these ...

Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies.

p.96-110 (2014) Keywords: Biomarkers Dna Female Genome, Human Genotype Humans Male polymerase chain reaction Polymorphism, Single Nucleotide public health Real-Time Polymerase Chain Reaction Receptors, ...

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