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DNase SISPA-next generation sequencing confirms Schmallenberg virus in Belgian field samples and identifies genetic variation in Europe.
generation sequencing. An in vitro transcribed RNA was used to construct a standard curve allowing the quantification of viral RNA in the field samples. Two field samples of aborted lambs containing 7.66 and ...
Fine quantification of avian influenza H5N1 escape mutant quasispecies populations using mutation-specific real-time PCR.
replication. The identification and quantification of these quasispecies populations require time-consuming and expensive cloning and sequencing approaches. In the present study, we developed mutation-specific ...
Quantification of foot-and-mouth disease virus transmission rates using published data.
Publication Type: Peer reviewed scientific article Authors: Goris, Nesya E; Eblé, Phaedra L; de Jong, Mart C M; Kris De Clercq Source: ALTEX, Volume 26, Issue 1, p.52-4 (2009) Keywords: Animal Testing Alternatives Animals Foot-and-Mouth Disease Foot-and-M ...
Technical note: Flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability.
procedure for the quantification of the proportion of viable, apoptotic, and necrotic polymorphonuclear neutrophilic leukocytes (PMNL) isolated from both low- and high-somatic-cell-count quarter milk samples. ...
A universal avian endogenous real-time reverse transcriptase-polymerase chain reaction control and its application to avian influenza diagnosis and quantification.
reverse transcriptase-polymerase chain reaction (RRT- PCR) is becoming an established first-line diagnostic assay as well as a precise quantification tool for avian influenza virus detection. However, there ...
Streptomycin as a selective agent to facilitate recovery and isolation of introduced and indigenous Sphingomonas from environmental samples.
A medium based on the aminoglycoside antibiotic streptomycin (Sm) was developed, which, together with the yellow pigmentation of Sphingomonas, facilitated the detection, recovery and quantification of ...