Search results - 26 results

Novel prediction models for genotoxicity based on biomarker genes in human HepaRG™ cells.

RNA-sequencing, was investigated. Overall, the RF and SVM models were complementary in their classification of chemicals for genotoxicity. To facilitate data analysis, an online application was developed, combining ...

Occurrence of microcystin congeners in surface waters and food in Belgium as a source of exposure to humans

the producers of these toxins by amplification and sequencing of the mcyE gene. Yet, data on the MC s prevalence is not sufficient to accurately assess human exposure and risk. Additional consumption ...

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

have always been analyzed and characterized after isolation. Since the advent of second generation sequencing, it has been possible to characterize the complete genome of these isolates to the SNP level. ...

Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard

Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Mathieu Gand; Indre Navickaite; Josephine Gruetzke; Lee-Julia Bartsch; Saria Otani; Giuliano Garofolo; Marco Di Domenico; Valeria Michel ...

Report on the feasibility of using Hi-C sequencing for metagenomics to define the context of AMR genes

Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Josephine Grützke; Carlus Deneke; Jennie Fischer; Lee Julia Bartsch; Bram Bloemen; Kevin Vanneste; Jolien D'aes; Nancy Roosens; Man ...

Optimised long-read sequence bioinformatics tool for the analysis of the resistome and microbial communities

https://doi.org/10.5281/zenodo.7433258 Keywords: Antimicrobial resistance bioinformatics Long read metagenomic sequencing Microbial community Resistome Health Topics:  Antimicrobial resistance (AMR) Food consumption and food safety ...

A Summer of Cyanobacterial Blooms in Belgian Waterbodies: Microcystin Quantification and Molecular Characterizations

method allowed to detect and quantify eight microcystin congeners. The mcyE gene was detected using PCR, while dominant cyanobacterial species were identified using 16S RNA amplification and direct ...

Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database

Publication Type: Peer reviewed scientific article Authors: Assia Saltykova; Julien Van Braekel; N. Papazova; Marie-Alice Fraiture; Deforce, Dieter; Kevin Vanneste; Sigrid C.J. De Keersmaecker; Nancy Roosens Source: Food Chemistry: Molecular Sciences, Vol ...

Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants

COVID-19 diagnosis is the detection of viral RNA by RT-qPCR with a specific set of primers and probe. It is important to frequently evaluate the performance of these tests and this can be done first by an in ...

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