Search results - 101 results

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

Nucleotide Real-Time Polymerase Chain Reaction RNA Replicase RNA, Viral Sensitivity and Specificity whole genome sequencing Abstract: The current CO ronaVIrus Disease 2019 (COVID-19) pandemic started in ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

outbreak, early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

a multiplex reverse transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, ...

Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate.

Influenza in Birds Poultry Diseases Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Viral Plaque Assay virulence Virus Shedding Abstract: The G1- H9N2 avian influenza virus (AIV) has caused ...

Seroprevalence of border disease virus and other pestiviruses in sheep in Algeria and associated risk factors.

Issue 1 (2018) Keywords: Algeria Animals Border Disease Border disease virus Enzyme-Linked Immunosorbent Assay Female Male Pestivirus Pestivirus Infections Real-Time Polymerase Chain Reaction Risk Factors ...

Hepatitis E virus serology and PCR: does the methodology matter?

Viral Hepatitis E Hepatitis E virus Humans Immunoglobulin G Immunoglobulin M polymerase chain reaction Sensitivity and Specificity Serologic Tests Abstract: Hepatitis E virus (HEV) genotype 3 is an ...

Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.

Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.

important. To avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. Among these molecular methods, the polymerase chain reaction (PCR) and ...

Detection and characterization of Brucella spp. in bovine milk in small-scale urban and peri-urban farming in Tajikistan.

Ulf Source: PLoS Negl Trop Dis, Volume 11, Issue 3, p.e0005367 (2017) Keywords: Agriculture Animals Brucella Cattle DNA, Bacterial DNA-Directed RNA Polymerases Humans milk prevalence Real-Time ...

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