Search results - 7 results
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika (ZIKV) ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.
transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, were selected from ...
Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different isolates. Genetic variation can however result in mismatches of primers and probes with their ...
Critical Aspects for Detection of Coxiella burnetii.
Keywords: Animals Bacteriological Techniques Coxiella burnetii Genome, Bacterial Humans Q Fever Real-Time Polymerase Chain Reaction Abstract: Coxiella burnetii is a globally distributed zoonotic ...
The epidemiology of subclinical malaria infections in South-East Asia: findings from cross-sectional surveys in Thailand-Myanmar border areas, Cambodia, and Vietnam.
a sensitive, high blood volume quantitative real-time polymerase chain reaction method in three countries of the Greater Mekong Sub-region. METHODS: Cross-sectional surveys were conducted in three villages in ...
Evaluation of 3 rapid influenza diagnostic tests during the 2012-2013 epidemic: influences of subtype and viral load.
H1N1 Subtype Influenza A Virus, H3N2 Subtype Influenza, Human Male middle aged Prospective Studies Real-Time Polymerase Chain Reaction Sensitivity and Specificity Viral Load Young adult Abstract: This ...
Assessment of pfcrt 72-76 haplotypes eight years after chloroquine withdrawal in Kinshasa, Democratic Republic of Congo.
collected in 2010 from a paediatric clinic in Kinshasa, DRC. Microscopy controls and real-time polymerase chain reaction (RT- PCR) were performed for Plasmodium species identification. Haplotypes of the pfcrt ...