Search results - 138 results

Selection of a Noninvasive Source of Human DNA Envisaging Genotyping Assays in Epidemiological Studies: Urine or Saliva?

Biomolecular Techniques: JBT, Volume 31, Issue 1 (2020) Keywords: Biomarkers children. genetic epidemiology Genotype Real-time PCR Abstract: Genetic epidemiology requires an appropriate approach to measure ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

outbreak, early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika ...

Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products

First, the potential presence of the AMR gene is detected by real-time PCR. Next, its full-length is evaluated by a nested- PCR amplifying a large fragment of its sequence to determine the risks of likely ...

Increased surface area of halloysite nanotubes due to surface modification predicts lung inflammation and acute phase response after pulmonary exposure in mice

of lung inflammation. The pulmonary and hepatic acute phase responses were assessed by Serumamyloida mRNA levels in lung and liver tissue by real-time quantitative PCR. Pulmonary and systemic ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

a multiplex reverse transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, ...

Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java.

biochemical tests. Therefore, a quadruplex real-time PCR method, based on the allelic discrimination of molecular markers selected from the scientific literature and from whole genome sequencing data produced ...

Seroprevalence of border disease virus and other pestiviruses in sheep in Algeria and associated risk factors.

Issue 1 (2018) Keywords: Algeria Animals Border Disease Border disease virus Enzyme-Linked Immunosorbent Assay Female Male Pestivirus Pestivirus Infections Real-Time Polymerase Chain Reaction Risk Factors ...

Clonal Spread of Candida glabrata Bloodstream Isolates and Fluconazole Resistance Affected by Prolonged Exposure: a 12-Year Single-Center Study in Belgium.

genetic profiles, and infected patients were hospitalized in the same unit during an overlapping period. Finally, quantitative real-time PCR analyses showed that, unlike that for other ABC efflux pumps, the ...

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification ...

Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system

Microbiology and Biotechnology, Volume 102, Issue 7, Number 3285 (2018) Keywords: Detection; Pathogenic E. coli; Real-time PCR; STEC; SYBR® Green; Validation Abstract: A detection and discrimination system for ...

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