Search results - 11 results
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison.
Véronique Duquesne; Severine Matthijs Source: Insects, Volume 13, Issue 1 (2021) Keywords: Aethina tumida Diagnosis honey bee inter-laboratory comparison Morphology Real-time PCR Small Hive Beetle Abstract: ...
Metagenomic sequencing determines complete infectious bronchitis virus (avian Gammacoronavirus) vaccine strain genomes and associated viromes in chicken clinical samples.
segment B closely related to D3976 /1 from Germany, 2017) and avian orthoreovirus, while three avian orthoavulavirus 1 reads confirmed prior real-time RT- PCR result. IBV sequence variation analysis ...
Detection of Clinical and Subclinical Lumpy Skin Disease Using Ear Notch Testing and Skin Biopsies.
subclinical, the sensitivity of clinical surveillance could be lower than expected. Furthermore, real-time PCR for the detection of LSD viral DNA in blood samples from subclinical animals is only intermittently ...
Retrospective survey of unauthorized genetically modified bacteria harbouring antimicrobial resistance genes in feed additive vitamin B2 commercialized in Belgium: Challenges and solutions
feed additive vitamin B2 products. To this end, two real-time PCR markers specific to this unauthorized genetically modified microorganism (GMM), named UGMV it- B2 and 558, were used. In the present ...
Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From Produced and Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer.
protocols used was successful in removing the viral genome completely from the produced and derived embryos as was demonstrated by real-time PCR. Moreover, BTV-8 virus was transmitted to recipient cows after ...
Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus
Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; David Lefebvre; Ilse De Leeuw; Steven Van Borm; Kris De Clercq Source: Journal of Virological Methods, Volume 246 (2017) Health Topics: Animal health Santé animale Diergezon ...
A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
p.e0164463 (2016) Keywords: Animals DNA Primers Foot-and-Mouth Disease Virus Humans Real-Time Polymerase Chain Reaction Abstract: Non-specific tail sequences are often added to the 5’-terminus of primers to ...
Application of a Commercial Real-Time RT-PCR Assay for Surveillance of Classical Swine Fever: Evaluation by Testing Sequential Tissue and Blood Samples
Koenen Source: The Open Veterinary Science Journal, Volume 2, Issue 1 (2008) Abstract: The applicability of the commercial TaqVet CSFV real-time RT- PCR assay (TaqVet CSF) as a diagnostic tool for herd ...
Comparative Analysis of Different Serological and Molecular Tests for the Detection of Small Ruminant Lentiviruses (SRLVs) in Belgian Sheep and Goats.
extensive comparative analysis of seven commercially available serological tests and one in-house real-time PCR (qPCR) detecting genotype A and B strains using a large panel of representative Belgian field ...
Nationwide Screening for Bee Viruses and Parasites in Belgian Honey Bees.
https://www.sciensano.be/en/biblio/nationwide-screening-bee-viruses-and-parasites-belgian-honey-bees
black queen cell virus (BQCV), and sacbrood virus (SBV) were highly prevalent and detected by real-time RT- PCR in more than 95% of the colonies. Acute bee paralysis virus (ABPV), chronic bee paralysis ...