Search results - 174 results
Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.
region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this ...
Evaluation of mycobacteria-specific gamma interferon and antibody responses before and after a single intradermal skin test in cattle naturally exposed to M. avium subsp. paratuberculosis and experimentally infected with M. bovis.
Interferon-gamma Release Tests Male Mycobacterium avium subsp. paratuberculosis Mycobacterium bovis Paratuberculosis Real-Time Polymerase Chain Reaction Tuberculin Test Tuberculosis, Bovine Abstract: This study ...
Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
Microbiology and Biotechnology, Volume 102, Issue 7, Number 3285 (2018) Keywords: Detection; Pathogenic E. coli; Real-time PCR; STEC; SYBR® Green; Validation Abstract: A detection and discrimination system for ...
A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods
International Journal of Food Microbiology (2018) Keywords: Biochemical methods Conventional PCR Oysters Prawns Real-time PCR seafood Vibrios Abstract: Globally, vibrios represent an important and ...
Development and validation of a TaqMan probe-based real-time PCR method for the differentiation of wild type lumpy skin disease virus from vaccine virus strains.
associated with adverse reactions resembling disease symptoms. The latter hampers clinical diagnosis and poses challenges in virus identification. To this end, a duplex quantitative real-time PCR method ...
Hepatitis E virus serology and PCR: does the methodology matter?
(Altona RealStar®), and two optimized in-house real-time RT- PCR assays (according to Jothikumar et al., 2006 and Gyarmati et al., 2007). In addition, all three PCR assays were performed on 16 external ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
and Specificity Sheep Swine Abstract: The 3D and 5UTR real-time RT- PCR assays (RT-qPCR) from Callahan et al. (2002) and Reid et al. (2002) are commonly used reference methods for the detection of ...
Brucella abortus is Prevalent in Both Humans and Animals in Bangladesh.
human sera and all animal samples were screened by Brucella genus-specific real-time PCR (RT- PCR), and positive samples were then tested by IS711 RT- PCR to detect B. abortus and B. melitensis DNA. Only ...
A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.
presented results demonstrate the potential of the modified Magnetic Capture real time PCR as a full alternative to the mouse bioassay for the screening of various types of tissues and meat, with the ...
Development of a real-time PCR test for the geno-serotyping of Salmonella Paratyphi B and its variant Java
Publication Type: Scientific poster, presentation or proceeding Authors: Mathieu Gand; Wesley Mattheus; Assia Saltykova; Nancy Roosens; Katelijne Dierick; Marchal, Kathleen; Sigrid C.J. De Keersmaecker; Sophie Bertrand Source: BSFM conference, BSFM, Bruss ...