Search results - 174 results
Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.
(PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if these tools have demonstrated their appropriate performance, some of them are not able to ...
Detection and characterization of Brucella spp. in bovine milk in small-scale urban and peri-urban farming in Tajikistan.
milk in the urban and peri-urban area of Dushanbe, Tajikistan. Brucella DNA was detected in 10.3% of 564 cow milk samples by IS711-based real-time PCR. This finding is concerning because consumption of ...
Incorporation of the influenza A virus NA segment into virions does not require cognate non-coding sequences.
incorporation of the viral segments was tested by real-time RT- PCR. An initial virus growth deficiency correlated to a specific defect in NA segment incorporation. Upon serial passages, growth properties were ...
Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
a specific ITS-based SYBR ®green real-time PCR (qPCR) assay for Aspergillus versicolor, a species frequently observed in indoor air and known to be allergenic. Additionally, an ITS-based qPCR assay was ...
Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus
Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; David Lefebvre; Ilse De Leeuw; Steven Van Borm; Kris De Clercq Source: Journal of Virological Methods, Volume 246 (2017) Health Topics: Animal health Santé animale Diergezon ...
Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator).
A Newcastle disease virus (NDV) was isolated in chicken embryonated eggs after detection by real-time reverse transcription- PCR (RRT- PCR) from a captive owl swab. The complete genome sequence of ...
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.
Reaction Reference Standards RNA, Messenger Abstract: Quantitative real-time PCR assays are widely used for the quantification of mRNA within avian experimental samples. Multiple stably-expressed reference ...
A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
p.e0164463 (2016) Keywords: Animals DNA Primers Foot-and-Mouth Disease Virus Humans Real-Time Polymerase Chain Reaction Abstract: Non-specific tail sequences are often added to the 5’-terminus of primers to ...
Clinical problems due to encephalomyocarditis virus infections in two pig herds.
conventional gel-based polymerase chain reaction tests. The real-time PCR test that gave initially negative result was further optimized to avoid false negative results. CONCLUSIONS: Typical lesions are not ...
Investigation on possible transmission of monkeys' Plasmodium to human in a populations living in the equatorial rainforest of the Democratic republic of Congo.
near the rainforest of the Democratic Republic of Congo (DRC) where several species of non-human primates are living. Two successive real-time PCR s were identified in the literature and used in ...