Search results - 174 results
Evaluation of detection methods for non-O157 Shiga toxin-producing Escherichia coli from food.
in parallel in Buffered Peptone Water (BPW) and Brila broth. After enrichment, detection was performed using real-time PCR (qPCR), and isolation using two chromogenic agar media, CHROM agar™ STEC and ...
A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay
jeanselmei. In this study, we developed a SYBR ®green real-time PCR assay based on the internal transcribed spacer 2 from the 18S ribosomal DNA complex for the specific detection of E. jeanselmei. The ...
Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.
Volume 15, p.103 (2015) Keywords: Bacillus subtilis genetic engineering High-Throughput Nucleotide Sequencing Organisms, Genetically Modified Real-Time Polymerase Chain Reaction riboflavin Sensitivity and ...
Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep.
euthanasia. The presence of SBV RNA in serum and organs was measured with quantitative real-time PCR. The appearance and persistence of neutralizing and SBV nucleoprotein (N) isotype specific antibodies was ...
Erratum to: Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
Publication Type: Peer reviewed scientific article Authors: Libert, X; Camille Chasseur; Bladt, S; Ann Packeu; Bureau, F; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Appl Microbiol Biotechnol, Volume 99, Issue 19, p.8319-20 (2015) Health Topics: F ...
Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
excellent alternative for the monitoring of indoor fungal contaminations. As a case study, we developed a SYBR ® green real-time PCR-based assay for the specific detection and identification of Aspergillus ...
First isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzania.
with the RBT. Three Brucella were isolated from an aborted fetus and associated membranes. Real time PCR (IS711), Bruce-ladder and classical biotyping classified the isolates as B. abortus biovar 3. ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing
number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...
European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples: The method of extraction is critical for SBV RNA detection in semen.
Bernd Source: J Vet Diagn Invest, Volume 27, Issue 4, p.422-30 (2015) Keywords: Animals Bunyaviridae Infections Cattle Cattle Diseases Europe Orthobunyavirus Real-Time Polymerase Chain Reaction Reverse ...
Detection and Isolation of Swine Influenza A Virus in Spiked Oral Fluid and Samples from Individually Housed, Experimentally Infected Pigs: Potential Role of Porcine Oral Fluid in Active Influenza A Virus Surveillance in Swine.
(2015) Keywords: Animals Chick Embryo Influenza A Virus, H1N1 Subtype Influenza A Virus, H3N2 Subtype Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Saliva Swine ...