Search results - 174 results
New trait-specific qualitative SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS
diverse. Consequently, the screening approaches have to be updated to enable full coverage and better discrimination of all these events. To respond to this need, two new qualitative SYBR ®Green real-time ...
Bluetongue virus RNA detection by real-time rt-PCR in post-vaccination samples from cattle.
positive using BTV-specific real-time RT- PCR, raising questions about a possible low level of virus circulation. An interference of the BTV-8 inactivated vaccine on the result of the real-time RT- PCR was ...
Chasing notifiable avian influenza in domestic poultry: a case report of low-pathogenic avian influenza h5 viruses in two Belgian holdings.
holding a low-pathogenic avian influenza (LPAI) virus subtype H5N2, and a H5 LPAI virus was identified by real-time PCR and direct sequencing at the second holding. The first farm was an outdoor mixed ...
Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies.
species-specific real-time quantitative (q) PCR assays, as DNA extracted from urine contains, besides human, microbial DNA also, which largely contributes to the total DNA yield. In addition, the kits giving a good ...
Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars.
wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT- PCR assays in an interlaboratory exercise are presented. Both assays ...
False-positive results in metagenomic virus discovery: a strong case for follow-up diagnosis.
32 757 sequences with average read length 335 bases) initially demonstrated the presence of parvovirus-like sequences in the tested blood sample. Thorough follow-up using specific real-time RT- PCR assays ...
Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis.
103, p.131-7 (2014) Keywords: Azides Microbial Viability Propidium Real-Time Polymerase Chain Reaction Salmonella enteritidis Salmonella Infections Sensitivity and Specificity Abstract: The propidium ...
Guidelines for validation of qualitative real-time PCR methods
modified organisms GMO GMO detection GMO screening Guidelines implement IS method methods ON PCR polymerase chain reaction qPCR qualitative real time PCR Real-time PCR Real-Time Polymerase Chain Reaction ...
Schmallenberg virus circulation in culicoides in Belgium in 2012: field validation of a real time RT-PCR approach to assess virus replication and dissemination in midges.
Marie; Ann Brigitte Cay Source: PLoS One, Volume 9, Issue 1, p.e87005 (2014) Keywords: Animals Belgium Bunyaviridae Infections Ceratopogonidae Insect Vectors Orthobunyavirus Real-Time Polymerase Chain ...
A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test.
two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT- PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species. Primers with 17 to ...