Search results - 174 results
A step forward in molecular diagnostics of lyssaviruses--results of a ring trial among European laboratories33977
laboratory. The ring trial allowed the important conclusion that conventional RT- PCR assays were really robust assays tested with a high concordance between different laboratories and assays. The real-time RT- ...
Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.
p.4021-37 (2013) Keywords: Bacterial Proteins Food Microbiology Listeria Listeria monocytogenes Real-Time Polymerase Chain Reaction Abstract: A combination of four qualitative SYBR ®Green qPCR screening ...
Diagnosis of Schmallenberg virus infection in malformed lambs and calves and first indications for virus clearance in the fetus.
Pregnancy Pregnancy Complications, Infectious Real-Time Polymerase Chain Reaction Sheep Sheep Diseases Sheep, Domestic Stillbirth Abstract: Since mid-December 2011, samples from malformed lambs and calves are ...
Viral load of high-risk human papillomaviruses as reliable clinical predictor for the presence of cervical lesions
Quantitative real-time PCR real time PCR Real-time PCR Research result results Sample Samples scale SCREENING SENSITIVITY specificity Test tests threshold thresholds transformation Type Viral Load Abstract: ...
New SYBR®Green methods targeting promoter sequences used for screening of several GM events pending for authorisation in Europe
events to be identified. To allow an extra covering power of the CoSYPS and to be able to include new EU-authorised GM events, two new SYBR ®Green real-time PCR (qPCR) methods targeting two promoter ...
What's in a strain? Viral metagenomics identifies genetic variation and contaminating circoviruses in laboratory isolates of pigeon paramyxovirus type 1.
four of these viral stocks. Real-time quantitative RT- PCR analysis specific for PMV-1 and PiCV showed that these contaminations were present in seven viral stocks consisting of allantoic fluids and was ...
Molecular detection and genotyping of noroviruses
detection of these viruses relies on the use of molecular methods such as (real-time) reverse transcriptase polymerase chain reaction (RT- PCR). Regardless of the matrix, detection of NoVs generally requires ...
Detection of Schmallenberg virus in different Culicoides spp. by real-time RT-PCR.
early autumn of 2011 at several places in Belgium by real-time RT- PCR. Pools of heads originating from following species: C. obsoletus complex, C. dewulfi and C. chiopterus were found positive, strongly ...
Evidence regarding human papillomavirus testing in secondary prevention of cervical cancer.
HC2, GP5 +/6+ PCR (polymerase chain reaction), cobas(®) 4800 PCR (Roche Molecular Systems Inc., Alameda, CA, USA) and Real Time PCR (Abbott Molecular, Des Plaines, IL, USA) can be considered as ...
Bluetongue sentinel surveillance program and cross-sectional serological survey in cattle in Belgium in 2010-2011.
real-time RT- PCR (RT-qPCR) were carried out in dairy cattle from the end of 2010 onwards with the aim to demonstrate the absence of BTV circulation in Belgium. This paper describes the results of the first ...