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Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different isolates. Genetic variation can however result in mismatches of primers and probes with their ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...
Different measles outbreaks in Belgium, January to June 2016- a challenge for public health.
Personnel hospitals Humans Immunoglobulin M Infant Infectious Disease Transmission, Patient-to-Professional Male Measles Measles Vaccine middle aged public health Reverse Transcriptase Polymerase Chain ...
Reconstruction of the Schmallenberg virus epidemic in Belgium: Complementary use of disease surveillance approaches.
Surveillance Reverse Transcriptase Polymerase Chain Reaction Seroepidemiologic Studies Abstract: Schmallenberg virus (SBV) emerged across Europe in 2011 and Belgium was among the first countries affected. In ...
European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples: The method of extraction is critical for SBV RNA detection in semen.
Bernd Source: J Vet Diagn Invest, Volume 27, Issue 4, p.422-30 (2015) Keywords: Animals Bunyaviridae Infections Cattle Cattle Diseases Europe Orthobunyavirus Real-Time Polymerase Chain Reaction Reverse ...
Experimental Infection of Sheep at 45 and 60 Days of Gestation with Schmallenberg Virus Readily Led to Placental Colonization without Causing Congenital Malformations.
Transmission, Vertical Male Orthobunyavirus Placenta Pregnancy Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sheep Sheep Diseases Time Factors Vero Cells Abstract: BACKGROUND: Main impact of ...
Detection and Isolation of Swine Influenza A Virus in Spiked Oral Fluid and Samples from Individually Housed, Experimentally Infected Pigs: Potential Role of Porcine Oral Fluid in Active Influenza A Virus Surveillance in Swine.
(2015) Keywords: Animals Chick Embryo Influenza A Virus, H1N1 Subtype Influenza A Virus, H3N2 Subtype Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Saliva Swine ...
Bluetongue virus RNA detection by real-time rt-PCR in post-vaccination samples from cattle.
p.157-62 (2015) Keywords: Animals Bluetongue Bluetongue virus Cattle Cattle Diseases Europe Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Vaccination ...
Diagnosis of the Lelystad strain of Porcine reproductive and respiratory syndrome virus infection in individually housed pigs: comparison between serum and oral fluid samples for viral nucleic acid and antibody detection.
Transcriptase Polymerase Chain Reaction Swine Abstract: There has been a developing interest in the use of oral fluid for the diagnosis of different pathogens such as Porcine reproductive and respiratory syndrome ...
No evidence of coronavirus infection by reverse transcriptase-PCR in bats in Belgium.
Thomas Source: J Wildl Dis, Volume 50, Issue 4, p.969-71 (2014) Keywords: Animals Belgium Chiroptera Coronavirus Coronavirus Infections Feces Reverse Transcriptase Polymerase Chain Reaction Abstract: No ...