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Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species.
isolation media. Nevertheless, already over 100 different species have been identified and characterized and their number increases as more hosts are sampled. We studied the applicability of amplified rDNA ...
The occurrence of encephalomyocarditis virus (EMCV) in European pigs from 1990 to 2001.
outbreaks were analysed and serum samples, partly from existing screening programmes, were tested for antibodies against EMCV. Most clinical EMCV outbreaks were reported in Belgium (320), followed by Italy ...
Real Time RT-PCR for the detection and quantitation of bovine respiratory syncytial virus.
the BRSV Real Time RT- PCR was then assessed by detecting BRSV in lungs, tracheas and bronchoalveaolar fluids (BAL) samples of experimentally infected calves. The assay was shown to be 100 times more ...
Population-level retrospective study of neurologically expressed disorders in ruminants before the onset of bovine spongiform encephalopathy (BSE) in Belgium, a BSE risk III country.
diagnosis. TSE analysis of a small, targeted historical sampling (n = 48) permitted the confirmation of one case and uncovered another case of scrapie. The results of the present study help to develop and ...
Streptomycin as a selective agent to facilitate recovery and isolation of introduced and indigenous Sphingomonas from environmental samples.
Publication Type: Peer reviewed scientific article Authors: Vanbroekhoven, Karolien; Ryngaert, Annemie; Bastiaens, Leen; P Wattiau; Vancanneyt, Marc; Swings, Jean; De Mot, René; Springael, Dirk Source: Environ Microbiol, Volume 6, Issue 11, p.1123-36 (200 ...
Acinetobacter diversity in environmental samples assessed by 16S rRNA gene PCR-DGGE fingerprinting.
to selectively amplify a fragment of the Acinetobacter 16S rRNA gene from environmental samples by PCR. 16S rRNA gene products were only obtained in PCR s with DNA from members of the genus ...
Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe.
Neutralization Tests Quality Control Reference Values Sensitivity and Specificity Vaccination Viral Proteins Viral Vaccines Abstract: Sets of serum and milk samples were collected from various countries and ...
Evaluation of virulence of Mycoplasma hyopneumoniae field isolates.
infection, pigs were euthanized, blood sampled and a lung lesion score was given. Lung samples were processed for histopathology, immunofluorescence testing for M. hyopneumoniae and isolation of M. ...
Real-time PCR for simultaneous detection and genotyping of bovine viral diarrhea virus.
isolates of both genotypes. The application to clinical diagnosis was evaluated on pooled blood samples by post run measurement of the FAM- and VIC-associated fluorescence. The 100% agreement with the ...
Dual labeling of Pseudomonas putida with fluorescent proteins for in situ monitoring of conjugal transfer of the TOL plasmid.
coexpressed in donor P. putida cells. Cells expressing both fluorescent proteins were smaller in size than cells expressing GFP alone. Donors and transconjugants in mixed culture or sludge samples were ...